Is stem cell chromosomes stability affected by cryopreservation conditions?
- 73 Downloads
The introduction of the recombinant DNA techniques in the 1970s paved the way to “Gene Therapy” a novel branch of modern medicine. Although gene therapy is still at an experimental stage, it has great potentials since allows to transfer, into organs and tissues, genetic information. However, several technical problems still need to be overcome before this approach takes over conventional pharmacological treatments (Hacein-Bey-Abina et al. 2002; Mullen et al. 1996).
Today stem cells, either embryonic or adult, are acquiring a great deal of attention as they promise, and rightly so, to be new vehicles for gene therapy. Unfortunately, the in vivo genetic instability of stem cells, and even more pronounced for embryonic derived stem cells, limits their widespread use. The prototypic example of adult stem cells, the hematopoietic stem cells have already been used in gene therapy (Aiuti et al. 2002) after being isolated from bone marrow or after their mobilization into...
KeywordsStem Cell Embryonic Stem Cell Trehalose Adult Stem Cell Sister Chromatid Exchange
We thank NEUROscreen (FP6, EU) for the valuable support in this research.
- Buchanan SS, Menze MA, Hand SC, Pyatt DW, Carpenter JF (2005) Cryopreservation of Human Hematopoietic Stem and Progenitor cells loaded with Trehalose:Transient permaeabilization via the adenosine triphosphate dependent P2Z receptor channel. Cell Preserv Technol 3:212–222. doi: 10.1089/cpt.2005.3.212 CrossRefGoogle Scholar
- Clapisson G, Salinas C, Malacher P, Michallet M, Philip I, Philip T (2004) Cryopreservation with hydroxyethylstarch (HES) + dimethylsulfoxide (DMSO) gives better results than DMSO alone. Bull Cancer 91:E97–E102Google Scholar
- Edwards MK, Harris JF, McBurney MW (1983) Induced muscle differentiation in an embryonal carcinoma cell line. Mol Cell Biol 3:2280–2286Google Scholar
- Inzunza J, Sahlen S, Holmberg K, Stromberg AM, Teerijoki H, Blennow E et al (2004) Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation. Mol Hum Reprod 10:461–466. doi: 10.1093/molehr/gah051 CrossRefGoogle Scholar
- Leibovitz BE, Siegel BV (1980) Aspects of free radical reactions in biological systems: aging. J Gerontol 35:45–56Google Scholar
- Mullen CA, Snitzer K, Culver KW, Morgan RA, Anderson WF, Blaese RM (1996) Molecular analysis of T lymphocyte-directed gene therapy for adenosine deaminase deficiency: long-term expression in vivo of genes introduced with a retroviral vector. Hum Gene Ther 7:1123–1129. doi: 10.1089/hum.1996.7.9-1123 CrossRefGoogle Scholar
- Pathak S, Multani AS (2006) Aneuploidy, stem cells and cancer. EXS 96:49–64Google Scholar
- Pera MF, Reubinoff B, Trounson A (2000) Human embryonic stem cells. J Cell Sci 113(Pt 1):5–10Google Scholar
- Stocum DL (2005) Stem cells in CNS and cardiac regeneration. Adv Biochem Eng Biotechnol 93:135–159Google Scholar