Cytotechnology

, Volume 56, Issue 3, pp 145–149

A method for measuring mitochondrial mass and activity

  • Maria Agnello
  • Giovanni Morici
  • Anna Maria Rinaldi
Brief Report

Abstract

Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.

Keywords

CLSM Mitochondrial mass Mitochondrial activity MitoTracker Green MitoTracker Orange Sea urchin 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Maria Agnello
    • 1
  • Giovanni Morici
    • 1
  • Anna Maria Rinaldi
    • 1
  1. 1.Dipartimento di Biologia Cellulare e dello Sviluppo “A. Monroy”Università degli Studi di PalermoPalermoItaly

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