Advertisement

Cytotechnology

, Volume 56, Issue 3, pp 171–178 | Cite as

Unidentified cells reside in fish skeletal muscle

  • M. V. DodsonEmail author
  • A. Kinkel
  • J. L. Vierck
  • K. Cain
  • M. Wick
  • J. Ottobre
Method in Cell Science

Abstract

Cell cultures were established from the skeletal muscle tissue of 6–13 months old rainbow trout and 12–14 months old yellow perch. Approximately 27,000 ± 5,000 cells/g (trout; N = 5) and 5,000 ± 1,200 cells/g of tissue (perch; N = 4) were obtained. Isolation and propagation were qualitatively greater for both species when the cells (younger fish producer more cells than older fish) were exposed to DMEM + 15% FBS, rather than L-15 + 15% FBS, at 20 °C (trout) and at 24 °C (yellow perch). Two morphologically distinct cell types were observed in cultures of both species, some of which eventually formed very small myotubes, which displayed immunocytological reactivity for myogenin, myosin heavy chain, and α-actinin; the second population of cells remained unstained. Successful cryopreservation was achieved using a 5% DMSO and 95% serum mixture, but post-thawing viabilities were low 5–27% (trout) and 14–30% (perch). Further research is needed in order to determine cell type specificity of isolated cells.

Keywords

Fish cells Yellow perch Rainbow trout Cell markers 

References

  1. Burton NM, Byrne KM, Vierck JL, Dodson MV (2000) Methods for satellite cell culture under a variety of conditions. Methods Cell Sci 22:51–61CrossRefGoogle Scholar
  2. Castillo J, Codina M, Martinez ML, Navarro I, Gutierrez J (2004) Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout. Am J Physiol Regul Integr Comp Physiol 286:R935–R941Google Scholar
  3. Dodson MV, McFarland DC, Grant AL, Doumit ME, Velleman SJ (1996) Invited review: extrinsic regulation of domestic animal-derived satellite cells. Domest Anim Endocrinol 13(2):107–126CrossRefGoogle Scholar
  4. Fauconneau B, Paboeuf G (2001) Sensitivity of muscle satellite cells to pollutants: an in vitro and in vivo comparative approach. Aquat Toxicol 53:247–263CrossRefGoogle Scholar
  5. Funkenstein B, Balas V, Skopal T, Radaelli G, Rowlerson A (2006) Long-term culture or muscle explants from Sparus aurata. Tissue Cell 38:399–415CrossRefGoogle Scholar
  6. Greenlee AR, Dodson MV, Yablonka-Reuveni Z, Kersten CA, Cloud JG (1995) In vitro differentiation of myoblasts from skeletal muscle of rainbow trout. J Fish Biol 46:731–747Google Scholar
  7. Malison JA (2000) A white paper on the status and needs of yellow perch aquaculture in the North Central region. North Central Regional Aquaculture Center, Michigan State University, East LansingGoogle Scholar
  8. Manci B (2000) Prospects for yellow perch aquaculture. Glob Aquac Advocate 3:62–63Google Scholar
  9. Matschak TW, Stickland NC (1995) The growth of Atlantic salmon (Salmo salar L.) myosatellite cells in culture at two different temperatures. Experientia 51:260–266CrossRefGoogle Scholar
  10. Mulvany DR, Cyrino JEP (1995) Establishment of channel catfish satellite cell cultures. Basic Appl Myol 5:65–70Google Scholar
  11. Powell R, Dodson M, Cloud J (1989) Cultivation and differentiation of satellite cells from skeletal muscle of the rainbow trout Salmo gairdneri. J Exp Zool 250:333–338CrossRefGoogle Scholar
  12. Pownall ME, Gustafsson MK, Emerson CP (2002) Myogenic regulatory factors and the specification of muscle progenitors in vertebrate embryos. Annu Rev Cell Dev Biol 18:747–783CrossRefGoogle Scholar
  13. Rice NA, Leinwand LA (2003) Skeletal myosin heavy chain function in cultured lung myofibroblasts. J Cell Biol 163:119–129CrossRefGoogle Scholar
  14. Thorgaard GH, Bailey GS, Williams D, Buhler DR, Kaattari SL, Ristow SS, Hansen JD, Winton JR, Bartholomew JL, Nagler JJ, Walsh PJ, Vijayan MM, Devlin RH, Hardy RW, Overturf KE, Young WP, Robison BD, Rexroad C, Palti Y (2002) Status and opportunities for genomics research with rainbow trout. Comp Biochem Physiol B 133:609–646CrossRefGoogle Scholar
  15. Van der Ven PF, Schaart G, Jap PH, Sengers RC, Stadhouders AM, Ramaekers FC (1992) Differentiation of human skeletal muscle cells in culture: maturation as indicated by titin and desmin striation. Cell Tissue Res 270:189–198CrossRefGoogle Scholar
  16. Venkateswaran V, Brackett EJ, Vierck J, Cloud JG, Dodson MV (1995) Substratum is an important determinant in growth factor regulation of trout-derived satellite cells. Basic Appl Myol 5:297–304Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • M. V. Dodson
    • 1
    Email author
  • A. Kinkel
    • 1
  • J. L. Vierck
    • 1
  • K. Cain
    • 2
  • M. Wick
    • 3
  • J. Ottobre
    • 3
  1. 1.Muscle Biology Laboratory, Department of Animal SciencesWashington State UniversityPullmanUSA
  2. 2.Department of Fish and Wildlife ResourcesUniversity of IdahoMoscowUSA
  3. 3.Department of Animal SciencesThe Ohio State UniversityColumbusUSA

Personalised recommendations