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Cytotechnology

, 57:115 | Cite as

Effects of polyamines on proliferation and IgM productivity of human–human hybridoma, HB4C5 cells

  • Takuya SugaharaEmail author
  • Sogo Nishimoto
  • Yoshiyuki Miyazaki
JAACT Special Issue

Abstract

Immunoglobulin production stimulating activity of polyamines was investigated. Spermidine, thermine and triethylenetetraamine (TETA) stimulated IgM production of human–human hybridoma, HB4C5 cells under serum-free condition. IgM production of HB4C5 cells was accelerated 5.9-, 5.3-, and 3.7-fold by spermidine at 4.5 mM, thermine at 2 mM and TETA at 2.5 mM, respectively. However, putrescine did not enhance IgM production. Spermidine enhanced IgM productivity of the hybridoma cells in spite of its growth suppression activity. TETA also inhibited cell proliferation and the effect on the acceleration of IgM productivity disappeared during 5 days because of its cytotoxicity. On the other hand, thermine facilitated IgM productivity of the hybridoma cells without growth suppression. The laser confocal microscopic analysis revealed that IgM content inside HB4C5 cells was increased by thermine. This result suggests that thermine facilitates IgM synthesis in hybridoma cells.

Keywords

Human–human hybridoma Immunoglobulin production stimulating factor Monoclonal antibody Polyamine Serum-free culture 

Abbreviations

BSA

Bovine serum albumin

ELISA

Enzyme-linked immunosorbent assay

FITC

Fluorescein isothiocyanate

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

Ig

Immunoglobulin

IPSF

Immunoglobulin production stimulating factor

PBS

Phosphate buffered saline

TETA

Triethylenetetraamine

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Takuya Sugahara
    • 1
    Email author
  • Sogo Nishimoto
    • 1
  • Yoshiyuki Miyazaki
    • 1
  1. 1.Faculty of AgricultureEhime UniversityMatsuyamaJapan

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