Cytotechnology

, Volume 56, Issue 1, pp 9–17

Morphology of fibroblasts grown on substrates formed by dielectrophoretically aligned carbon nanotubes

  • Felix L.-Y. Yuen
  • Gene Zak
  • Stephen D. Waldman
  • Aristides Docoslis
Brief Report

DOI: 10.1007/s10616-007-9113-0

Cite this article as:
Yuen, F.LY., Zak, G., Waldman, S.D. et al. Cytotechnology (2008) 56: 9. doi:10.1007/s10616-007-9113-0

Abstract

Multiwall carbon nanotube templates formed on the surfaces of planar interdigitated microelectrode arrays by means of AC electric field-guided assembly are being explored as potential substrates for tissue engineering. The objective of the present study is to examine whether surface patterns of aligned multiwall carbon nanotubes can have an effect on cell growth, morphology, and alignment. Bovine fibroblasts grown on aligned carbon nanotubes for a period of 2 weeks were found to have raised bodies and pronounced cell extensions for anchoring themselves to the substrate similar to that of the cells found in native tissues. On the other hand, cells grown on various control surfaces had a flat, circular morphology. The cell cultures were visualized by means of SEM imaging and the resulting morphologies were statistically analyzed and compared.

Keywords

AC electrokinetics Carbon nanotube alignment Dielectrophoresis Microelectrodes Patterned substrate Tissue engineering 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Felix L.-Y. Yuen
    • 1
  • Gene Zak
    • 2
  • Stephen D. Waldman
    • 1
    • 2
    • 3
  • Aristides Docoslis
    • 1
    • 3
  1. 1.Department of Chemical EngineeringQueen’s University at KingstonKingstonCanada
  2. 2.Department of Mechanical & Materials EngineeringQueen’s University at KingstonKingstonCanada
  3. 3.Kingston General HospitalKingstonCanada

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