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Cytotechnology

, Volume 52, Issue 2, pp 139–149 | Cite as

Use of cyanobacterial gas vesicles as oxygen carriers in cell culture

  • Anand Sundararajan
  • Lu-Kwang JuEmail author
Original Research

Abstract

The gas vesicles isolated from the cells of filamentous cyanobacterium Anabaena flos-aquae were treated and sterilized with glutaraldehyde and then evaluated for their effectiveness as gas carriers in cell culture. Anchorage-dependent Vero cells were grown in a packed bed of microcarrier beads under the perfusion of Dulbecco’s Modified Eagle’s Medium with 1% serum. The culture medium supplemented with 1.8% (v/v) gas vesicles was found to support a 30% higher maximum glucose utilization rate than the same medium without gas vesicles. The gas vesicle suspension was confirmed to have no apparent effects on cell metabolism in T-flask cultures. The study results indicated that the gas vesicles, with high oxygen carrying capacity, can be used to increase the oxygen supply in cell culture systems.

Keywords

Cyanobacteria Flocculation Gas vesicle Microcarrier culture Oxygen supply Vero cells 

Notes

Acknowledgements

The work was supported by a grant to Ohio Bioprocessing Research Consortium from Ohio Board of Regents and a Faculty Research Grant from The University of Akron.

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringThe University of AkronAkronUSA
  2. 2.Martek Biosciences Corp.WinchesterUSA

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