NucleoCounter—An efficient technique for the determination of cell number and viability in animal cell culture processes
- 279 Downloads
The NucleoCounter is a novel, portable cell counting device based on the principle of fluorescence microscopy. The present work establishes its use with animal cells and checks its reliability, consistency and accuracy in comparison with other cytometric techniques. The main advantages of this technique are its ability to handle a large number of samples with a high degree of precision and its simplicity and specificity in detecting viable cells quantitatively in a heterogeneous culture. The work addresses and overcomes the problems of subjectivity, and some of the inherent sampling errors associated with using the traditional haemocytometer and Trypan Blue exclusion method. NucleoCounter offers reduced intra- and inter-observer variation as well as consistency in repetitive analysis that establishes it as an efficient and highly potential device for at-line monitoring of animal cell processes. Furthermore, since the only manual steps required are sample aspiration and mixing with two reagents, it is feasible that the whole method could be automated and brought on-line for process monitoring and control.
KeywordsNucleoCounter Cell culture Monitoring Flow cytometry Cell count
Unable to display preview. Download preview PDF.
- Al-Rubeai M, Welzenbach K, Lloyd DR, Emery AN (1997) A rapid method for evaluation of cell number and viability by flow cytometry Cytotechnology. 24:161–168Google Scholar
- Butler M (1999) Cell counting and viability measurements. In: Jenkins N (eds) Animal cell biotechnology. Humana Press, NJ, pp 90–90Google Scholar
- Falkenhain A, Lorenz TH, Behrendt U, Lehmann J (1998) Dead cell estimation – a comparison of different methods. New developments and new applications in animal cell technology. Kluwer Academic Publishers, Amsterdam, pp 333–336Google Scholar
- Kwok SM, Ham W, McGowan MA, Ohashi R, Otero JM, Hamel JFP (2002) Evaluation of the automated cell density examination system (Cedex®). 224th ACS National meeting in Boston, USAGoogle Scholar
- Mukwena NT, Veraitch F, Santhalingham S, Al-Rubeai M, Goix P (2003) At-line monitoring of cell cultures: Rapid cytometric evaluation of cellular physiology. Guava Technologies, Application note, pp 1–7Google Scholar
- Winkelmeier P, Glauner B, Lindl T (1993) Quantification of cytoxicity by cell volume and cell proliferation. Atla 21:269–280Google Scholar