Cytotechnology

, Volume 51, Issue 1, pp 7–19

Automated time-lapse microscopy and high-resolution tracking of cell migration

  • Joseph S. Fotos
  • Vivek P. Patel
  • Norman J. Karin
  • Murali K. Temburni
  • John T. Koh
  • Deni S. Galileo
Original Paper

Abstract

We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from closely spaced time points (minutes), (2) over long periods (hours to days), (3) from multiple areas of interest, (4) in parallel under several different experimental conditions. Quantitation of true individual and average cell velocity and path length was obtained with high spatial and temporal resolution in “scratch” or “wound healing” assays. This revealed unique motility dynamics of drug-treated and adhesion molecule-transfected cells and, thus, this is a considerable improvement over current methods of measurement and analysis. Several fluorescent vital labeling methods commonly used for end-point analyses (GFP expression, DiO lipophilic dye, and Qtracker nanocrystals) were found to be useful for time-lapse studies under specific conditions that are described. To illustrate one application, fluorescently labeled tumor cells were seeded onto cell monolayers expressing ectopic adhesion molecules, and this resulted in consistently reduced tumor cell migration velocities. These highly quantitative time-lapse analysis methods will promote the creation of new cell motility assays and increase the resolution and accuracy of existing assays.

Keywords

Cell migration Green fluorescent protein Scratch assay Time-lapse Tumor cell lines Vital fluorescent labeling 

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Joseph S. Fotos
    • 1
  • Vivek P. Patel
    • 1
  • Norman J. Karin
    • 1
    • 3
  • Murali K. Temburni
    • 1
    • 2
  • John T. Koh
    • 2
  • Deni S. Galileo
    • 1
  1. 1.Department of Biological SciencesUniversity of DelawareNewarkUSA
  2. 2.Chemistry and BiochemistryUniversity of DelawareNewarkUSA
  3. 3.Pacific Northwest National LaboratoryRichlandUSA

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