Cytotechnology

, Volume 48, Issue 1–3, pp 89–95

Effect of Serum Replacement with Plysate on Cell Growth and Metabolismin Primary Cultures of Human Skeletal Muscle

  • David K Krämer
  • Karim Bouzakri
  • Olle Holmqvist
  • Lubna Al-Khalili
  • Anna Krook
Article

Abstract

Cell- and tissue culture methodology form an important base for biological, biochemical and biomedical research. Most cell culture techniques require the use of animal sera for the successful propagation of cells. However, the varying composition between batches has fuelled the need for alternatives. In the case of serum derived from animal foetuses, ethical concerns have also been raised. Here we compare the use of a platelet derived lysate (Plysate), which is currently under development as a serum substitute, in the culturing of primary human muscle cells to foetal bovine serum (FBS). In cells cultured with Plysate, differentiation into myotubes, glucose-uptake, phosphatidylinositol 3-kinase (PI3K) activity and expression and phosphorylation of ERK1/2 MAPK and PKB/Akt was impaired. Thus for primary human skeletal muscle Plysate is a sub-optimal substitute for FBS.

Keywords

Foetal bovine serum (FBS) Insulin action Platelet derived lysate (Plysate) Primary human skeletal muscle cell culture Serum replacement 

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Copyright information

© Springer 2005

Authors and Affiliations

  • David K Krämer
    • 1
    • 2
  • Karim Bouzakri
    • 1
  • Olle Holmqvist
    • 3
  • Lubna Al-Khalili
    • 1
    • 2
  • Anna Krook
    • 2
  1. 1.Department of Surgical ScienceKarolinska InstitutetStockholmSweden
  2. 2.Department of Physiology and PharmacologyKarolinska InstitutetStockholmSweden
  3. 3.ProLiff ABKalmarSweden

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