, Volume 46, Issue 1, pp 9–18 | Cite as

Identification of three gene candidates for multicellular resistance in colon carcinoma

  • Nicholas E. Timmins
  • Tina L. Maguire
  • Sean M. Grimmond
  • Lars K. Nielsen


Solid tumours display elevated resistance to chemo- and radiotherapies compared to individual tumour derived cells. This so-called multicellular resistance (MCR) phenomenon can only be partly explained by reduced diffusion and altered cell cycle status; even fast growing cells on the surface of solid tumours display MCR. Multicellular spheroids (MCS) recapture this phenomenon ex vivo and here we compare gene expression in exponentially growing MCS with gene expression in monolayer culture. Using an 18,664 gene microarray, we identified 42 differentially expressed genes and three of these genes can be linked to potential mechanisms of MCR. A group of interferon response genes were also up-regulated in MCS, as were a number of genes that that are indicative of greater differentiation in three-dimensional cultures.


3D culture Differential gene expression MCR MCS Multicellular resistance Multicellular spheroid Tumour 







Multicellular resistance


Multicellular spheroid


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Copyright information

© Springer 2005

Authors and Affiliations

  • Nicholas E. Timmins
    • 1
  • Tina L. Maguire
    • 2
  • Sean M. Grimmond
    • 2
  • Lars K. Nielsen
    • 1
  1. 1.Laboratory for Biological Engineering, Department of Chemical EngineeringUniversity of QueenslandBrisbaneAustralia
  2. 2.The Institute for Molecular BiosciencesThe University of QueenslandBrisbaneAustralia

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