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Chemistry of Natural Compounds

, Volume 55, Issue 6, pp 1123–1126 | Cite as

Accumulation and Excretion Dynamics and Purification of Lipase from Bacteria Pseudomonas aeruginosa A12 Culture Medium

  • N. Sh. BerdievEmail author
  • Zh. F. Ziyavitdinov
  • S. S. Bozorov
  • U. Zh. Ishimov
  • Sh. S. Olimzhonov
  • Sh. Zh. Fazliddinov
  • Sh. I. Salikhov
Article
  • 8 Downloads

A highly active and electrophoretically homogeneous enzyme with MM 31 kDa and specific lipase activity 1009 U/mg was isolated and purified from bacteria Pseudomonas aeruginosa A12 culture medium using ion-exchange and hydrophobic chromatography. The physicochemical properties of the isolated lipase were studied.

Keywords

Pseudomonas aeruginosa lipase bacteria activity chromatography 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • N. Sh. Berdiev
    • 1
    Email author
  • Zh. F. Ziyavitdinov
    • 1
  • S. S. Bozorov
    • 1
    • 2
  • U. Zh. Ishimov
    • 1
  • Sh. S. Olimzhonov
    • 1
  • Sh. Zh. Fazliddinov
    • 2
  • Sh. I. Salikhov
    • 1
  1. 1.A. S. Sadykov Institute of Bioorganic ChemistryAcademy of Sciences of the Republic of UzbekistanTashkentUzbekistan
  2. 2.Center of Advanced TechnologiesMinistry of Innovative DevelopmentTashkentUzbekistan

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