Steroids from Dendronephthya mucronata and Their Inhibitory Effects on Lipopolysaccharide-Induced No Formation in RAW264.7 Cells
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Four pregnane-type steroids including the new compound 5-α-pregn-20-en-3,6-dione (1) and two known cholestane-type steroids were isolated from the MeOH extract of the octocoral Dendronephthya mucronata. The structures of the compounds were elucidated using spectral data (PMR and 13C NMR, HSQC, HMBC, 1H−1H COSY, NOESY, HR-QTOF-MS) and comparisons with previously published information. The steroids exhibited moderate or weak inhibitory effects on inflammatory lipopolysaccharide-induced NO formation in RAW264.7 murine macrophage cells.
KeywordsDendronephthya mucronata Nephtheidae steroid NO formation
The studies were financially supported by grants of the Vietnam Academy of Science and Technology (QTRU01.04/18-19 and QTRU04.04/18-19) and a grant from the Russian Foundation for Basic Research No. 18-53-54002 V′et_a.
We thank the Institute of Chemistry, VAST, for recording NMR spectra and Dr. Bui Huu Tai (Institute of Marine Biochemistry, VAST) for taking the HR-QTOF mass spectra.
- 1.J. M. Kornprobst, Cnidaria and Ctenophora-1, in: Encyclopedia of Marine Natural Products, Wiley-VCH Verlag GmbH & Co. KGaA, 2014, p. 1087.Google Scholar