Chemical Constituents and Bioactivity of a Fungal Endophyte from Lamium amplexicaule
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Endophytic fungi are microorganisms that inhabit the intercellular spaces of plant stems, petioles, roots, and leaves without causing visible disease symptoms . Recently, many bioactive compounds with antitumor, antimicrobial, and antituberculosis properties were isolated from secondary metabolites of fungal endophytes . In this paper, we described the isolation and identification of 12 compounds (1–12) from an endophytic fungus BGC-2 (identified as Alternaria alternata) from Lamium amplexicaule. The fungal strain (BGC-2) was isolated from the stems of Lamium amplexicaule and identified as Alternaria alternata based on both morphology on PDA and analysis of the DNA sequences of the ITS1-5.8S-ITS2 ribosomal RNA gene region. A GenBank search for DNA sequence similarity revealed that ITS1-5.8S-ITS2 of BGC-2 was 99% homologous to that of Alternaria alternata reference strains (KU324787.1).
The endophytic fungus BGC-2 was cultivated in PDA medium (50 L) for 5 days at 28°C in a...
This work was supported by the National Natural Science Foundation of China (No. 21762027) and the Project of Science and Technology Department of Gansu Province, China (No. 1604FKCA084).