Chemistry of Natural Compounds

, Volume 53, Issue 5, pp 988–990 | Cite as

Lupane Triterpenoids and Sterols from Inonotus rheades Mycelium and their Anti-Glucosidase Activity

  • D. N. Olennikov
  • T. G. Gornostai
  • T. A. Penzina
  • G. B. Borovskii

Extracts and pure compounds from Inonotus obliquus are known to possess hypoglycemic activity due to inhibitory effects on several digestive enzymes in addition to antioxidant [1] and reparative action on pancreatic β-cells [2]. Previously, we found that extracts of I. rheades mycelium possessed antioxidant activity [3] due to the presence of phenolic compounds, in particular, styrylpyrones and bis(styrylpyrones) [4]. Studies of the biological activity of constituents from I. rheades mycelium established inhibitory activity of the plant extracts on several digestive enzymes. In particular, the 50% inhibition concentration for α-glucosidase activity (IC50) of hexane fraction Ir-01 was 56.40 μg/mL [4], which prompted detailed studies of it.

In order to determine the active compounds, fraction Ir-01 (0.5 g) was separated by column chromatography (CC) over silica gel (3 × 30 cm, hexane–EtOAc, 100:0→70:30) to produce subfractions Ir-01/1–Ir-01/7. Then, subfraction Ir-01/2 was...



The work was supported financially by the RFBR No. 16-43-030857.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • D. N. Olennikov
    • 1
  • T. G. Gornostai
    • 2
  • T. A. Penzina
    • 3
  • G. B. Borovskii
    • 2
  1. 1.Institute of General and Experimental Biology, Siberian BranchRussian Academy of SciencesUlan-UdeRussia
  2. 2.Siberian Institute of Plant Physiology and Biochemistry, Siberian BranchRussian Academy of SciencesIrkutskRussia
  3. 3.Research Institute of BiologyIrkutsk State UniversityIrkutskRussia

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