Lupane Triterpenoids and Sterols from Inonotus rheades Mycelium and their Anti-Glucosidase Activity
Extracts and pure compounds from Inonotus obliquus are known to possess hypoglycemic activity due to inhibitory effects on several digestive enzymes in addition to antioxidant  and reparative action on pancreatic β-cells . Previously, we found that extracts of I. rheades mycelium possessed antioxidant activity  due to the presence of phenolic compounds, in particular, styrylpyrones and bis(styrylpyrones) . Studies of the biological activity of constituents from I. rheades mycelium established inhibitory activity of the plant extracts on several digestive enzymes. In particular, the 50% inhibition concentration for α-glucosidase activity (IC50) of hexane fraction Ir-01 was 56.40 μg/mL , which prompted detailed studies of it.
In order to determine the active compounds, fraction Ir-01 (0.5 g) was separated by column chromatography (CC) over silica gel (3 × 30 cm, hexane–EtOAc, 100:0→70:30) to produce subfractions Ir-01/1–Ir-01/7. Then, subfraction Ir-01/2 was...
The work was supported financially by the RFBR No. 16-43-030857.
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