Polysaccharides of Fabaceae. VI. Galactomannans from seeds of Astragalus alpinus and A. tibetanus

Article
First Online:
Received:

Galactomannans with galactose:mannose ratios 1:1.48 and 1:1.33, [α]D +67.9 and +76.4°, [η] 870.3 and 1337.1 mL/g, and molecular weights 999 and 1549 kDa, respectively, were isolated in 0.59 and 4.65% yields (of seed mass) from seeds of Astragalus alpinus and A. tibetanus (Fabaceae). Physicochemical methods (CrO3 oxidation; methylation–GC/MS; IR, NMR, and 13C spectroscopy) found that the main polysaccharide chain consisted of 1,4-β-D-mannopyranose units substituted 67.5% (A. alpinus) and 75.2% (A. tibetanus) at the C-6 position by single α -D-galactopyranose units. The contents of mannobiose blocks Man–Man, (Gal)Man–Man/Man–Man(Gal), and (Gal)Man–Man(Gal) variously substituted with galactose were according to 13C NMR spectroscopy 15.9, 55.5, and 28.6% in A. alpinus galactomannan and 9.9, 42.3, and 47.8% in A. tibetanus galactomannan.

Keywords

Astragalus alpinus A. tibetanus Fabaceae galactomannans 13C NMR spectroscopy 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgment

The work was supported financially by the Lavrent´ev Competition for Young Scientists of the SB RAS.

References

  1. 1.
    D. N. Olennikov and A. V. Rokhin, Chem. Nat. Comp., 44, 685 (2008).CrossRefGoogle Scholar
  2. 2.
    D. N. Olennikov and A. V. Rokhin, Chem. Nat. Comp., 45, 297 (2009).CrossRefGoogle Scholar
  3. 3.
    D. N. Olennikov and A. V. Rokhin, Chem. Nat. Comp., 46, 165 (2010).CrossRefGoogle Scholar
  4. 4.
    D. N. Olennikov, I. Yu. Selyutina, and A. V. Rokhin, Chem. Nat. Comp., 46, 673 (2010).CrossRefGoogle Scholar
  5. 5.
    D. N. Olennikov and A. V. Rokhin, Appl. Biochem. Microbiol., 46, 113 (2010).Google Scholar
  6. 6.
    D. N. Olennikov and A. V. Rokhin, Appl. Biochem. Microbiol., 46, 444 (2010).CrossRefGoogle Scholar
  7. 7.
    D. N. Olennikov and A. V. Rokhin, Appl. Biochem. Microbiol., 46, 540 (2010).CrossRefGoogle Scholar
  8. 8.
    R. Ya. Plennik, Sib. Ekol. Zh., 2, 281 (2007).Google Scholar
  9. 9.
    I. E. Lobanova, O. V. Anulov, and V. D. Shcherbukhin, Turczaninowia, 10, 72 (2007).Google Scholar
  10. 10.
    H. L .Tookey, R. L. Lohmar, I. A. Wolff, and Q. Jones, J. Agric. Food Chem., 10, 131 (1962).CrossRefGoogle Scholar
  11. 11.
    O. V. Anulov, N. I. Smirnova, N. M. Mestechkina, I. A. Shreter, and V. D. Shcherbukhin, Prikl. Biokhim. Mikrobiol., 31, 645 (1995).Google Scholar
  12. 12.
    N. I. Smirnova, I. E. Lobanova, and O. V. Anulov, Rastit. Resur., 33, No. 4, 68 (1998).Google Scholar
  13. 13.
    N. M. Mestechkina, O. V. Anulov, N. I. Smirnova, and V. D. Shcherbukhin, Appl. Biochem. Microbiol., 36, 582 (2000).CrossRefGoogle Scholar
  14. 14.
    K. Kato, M. Nita, and T. Mizuno, Agric. Biol. Chem., 37, 433 (1973).CrossRefGoogle Scholar
  15. 15.
    S. Boziek, M. Izzard, and A. Morrison, Carbohydr. Res., 93, 279 (1981).CrossRefGoogle Scholar
  16. 16.
    V. D. Shcherbukhin and O. V. Anulov, Prikl. Biokhim. Mikrobiol., 35, 257 (1999).Google Scholar
  17. 17.
    Methods of Carbohydrate Chemistry [in Russian], Moscow, 1967, p. 286.Google Scholar
  18. 18.
    A. A. Mohamed and P. Rayas-Duarte, Cereal Chem., 72, 648 (1995).Google Scholar
  19. 19.
    N. M. Mestechkina, O. V. Anulov, N. I. Smirnova, and V. D. Shcherbukhin, Prikl. Biokhim. Mikrobiol., 32, 656 (1996).Google Scholar
  20. 20.
    N. I. Smirnova and V. D. Shcherbukhin, Prikl. Biokhim. Mikrobiol., 24, 653 (1988).Google Scholar
  21. 21.
    O. Ishurd, A. Kermagi, F. Zgheel, M. Flefla, M. Elmabruk, W. Yalin, J. F. Kennedy, and P. Yuanjiang, Carbohydr. Polym., 58, 41 (2004).CrossRefGoogle Scholar
  22. 22.
    I. Ciukanu and F. Kerek, Carbohydr. Res., 131, 209 (1984).CrossRefGoogle Scholar
  23. 23.
    D. N. Olennikov and L. M. Tankhaeva, Chem. Nat. Comp., 43, 501 (2007).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  1. 1.Institute of General and Experimental Biology, Siberian Branch, Russian Academy of SciencesUlan-UdeRussia
  2. 2.Irkutsk State UniversityIrkutskRussia

Personalised recommendations