Isolation and characteristics of highly active α-amylase from Bacillus subtilis-150
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Partially purified enzyme preparation with specific activities of 153.7 U/mg for α-amylase and 0.15 U/mg for protease was produced by selective adsorption on starch. Enzymes were purified until homogeneous electrophoretically by gel-filtration over HW-55 TSK-gel with specific activities of 245 U/mg for α-amylase and 1.44 U/mg for protease. The optimum temperature and pH for purified α-amylase activity are 40–50°C and pH 6.0. The effects of various metal ions on the activity and stability of the enzyme were studied.
Key wordsBacillus subtilis-150 α-amylase protease enzyme preparation purification properties enzyme activity
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