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Conservation Genetics

, Volume 19, Issue 6, pp 1393–1399 | Cite as

DNA barcoding of traded shark fins, meat and mobulid gill plates in Singapore uncovers numerous threatened species

  • Benjamin J. Wainwright
  • Yin Cheong Aden Ip
  • Mei Lin Neo
  • Jia Jin Marc Chang
  • Chester Zhikai Gan
  • Naomi Clark-Shen
  • Danwei Huang
  • Madhu Rao
Research Article

Abstract

The shark and ray (Elasmobranchii) trade is a commercially valuable industry that has negative consequences for wild populations. An estimated 100 million sharks are caught each year to supply the demand for cultural cuisines, traditions and practices, including shark fin soup and Traditional Chinese Medicine. Despite the establishment of frameworks and regulations by international trade and conservation bodies as well as national legislations, elasmobranch populations continue to decline. While their conservation becomes an ever more pressing concern, a major obstacle that hampers regulation is the mislabelling and/or misidentification of dried products or carcasses that have had fins removed. Here we use DNA barcoding to identify the species of origin for a variety of shark and ray products readily available to consumers in Singapore, a major importer of these goods. We amplified a fragment of the cytochrome c oxidase subunit I gene from shark fin, cartilage and meat, as well as ray gill plates and meat for DNA sequencing. Our analysis of 207 DNA barcodes yielded 28 positively identified elasmobranch species, eight of which are listed under Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, and 12 are listed as Endangered or Vulnerable by the International Union for the Conservation of Nature. This information will be useful to regulatory bodies in controlling trade and establishing new or revisiting previous conservation status listings.

Keywords

Singapore CITES IUCN Conservation Shark fin Gill plates 

Notes

Acknowledgements

Funding was generously provided by the Singapore Ministry of Education Academic Research Fund Tier 1 (R-154-000-A63-114) and the National Research Foundation, Prime Minister’s Office, Singapore under its Marine Science R&D Programme (MSRDP-P03). Financial support from the Wildlife Conservation Society is gratefully acknowledged.

Supplementary material

10592_2018_1108_MOESM1_ESM.xlsx (39 kb)
Supplementary material 1 (XLSX 40 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Benjamin J. Wainwright
    • 1
  • Yin Cheong Aden Ip
    • 1
  • Mei Lin Neo
    • 1
    • 2
  • Jia Jin Marc Chang
    • 1
  • Chester Zhikai Gan
  • Naomi Clark-Shen
  • Danwei Huang
    • 1
    • 2
  • Madhu Rao
    • 1
    • 3
  1. 1.Department of Biological SciencesNational University of SingaporeSingaporeSingapore
  2. 2.Tropical Marine Science InstituteNational University of SingaporeSingaporeSingapore
  3. 3.Wildlife Conservation SocietySingaporeSingapore

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