Conservation Genetics

, Volume 18, Issue 3, pp 573–584 | Cite as

Microsatellite analysis supports the existence of three cryptic species within the bumble bee Bombus lucorum sensu lato

  • Lorraine McKendrick
  • Jim Provan
  • Úna Fitzpatrick
  • Mark J. F. Brown
  • Tómas E. Murray
  • Eckart Stolle
  • Robert J. Paxton
Research Article

Abstract

Mitochondrial cytochrome oxidase I (COI) partial sequences are widely used in taxonomy for species identification. Increasingly, these sequence identities are combined with modelling approaches to delineate species. Yet the validity of species delineation based on such DNA ‘barcodes’ is rarely tested and may be called into question by phenomena such as ancestral polymorphisms in DNA sequences, phylogeographic divergence, mitochondrial introgression and hybridization, or distortion of mitochondrial inheritance through such factors as Wolbachia infection. The common and widespread European bumble bee Bombus lucorum s. lato contains three distinct mitochondrial DNA lineages that are assumed to represent three cryptic species, namely Bombus cryptarum, B. lucorum s. str. and B. magnus. To test whether nuclear gene pools of the three putative species were differentiated, we genotyped 304 sympatric members of the lucorum complex (54 B. cryptarum females, 168 B. lucorum s. str. females and 82 B. magnus females, as defined using mtDNA COI haplotypes) from 11 localities spread across the island of Ireland at seven nuclear microsatellite loci. Multilocus genotypes clustered into three discrete groups that largely corresponded to the three mtDNA lineages: B. cryptarum, B. lucorum s. str. and B. magnus. The good fit of mitochondrial haplotype to nuclear (microsatellite) genotypic data supports the view that these three bumble bee taxa are reproductively isolated species, as well as providing a vindication of species identity using so-called DNA barcodes.

Keywords

DNA barcode Cryptarum Magnus Mitochondrial cytochrome oxidase I Structure software PCoA DAPC Sympatry 

Notes

Acknowledgements

We thank friends and colleagues who helped to collect bumble bees across Ireland: D. Cookson, D. Dominoni, M. Kelly and S. Roos; Andreas Bertsch for use of his photographs, comments on this manuscript and encouragement to engage with the lucorum complex; and Robin Moritz for laboratory and intellectual support. We also thank two anonymous reviewers and editor-in-chief as well as Shalene Jha and Christophe Praz for many insightful comments that helped improve the manuscript.

Funding

This work was supported by a grant from the Higher Education Authority of Ireland as part of its North–South Research Programme for Peace and Reconciliation. L McKendrick thanks DARD for their financial support (a PhD stipend) and patience.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10592_2017_965_MOESM1_ESM.pdf (949 kb)
Supplementary material 1 (PDF 949 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Lorraine McKendrick
    • 1
  • Jim Provan
    • 2
  • Úna Fitzpatrick
    • 3
  • Mark J. F. Brown
    • 4
  • Tómas E. Murray
    • 3
  • Eckart Stolle
    • 5
  • Robert J. Paxton
    • 1
    • 6
    • 7
  1. 1.School of Biological SciencesQueen’s University BelfastBelfastUK
  2. 2.Institute of Biological, Environmental and Rural SciencesPenglais, Aberystwyth UniversityAberystwythUK
  3. 3.National Biodiversity Data CentreWaterfordIreland
  4. 4.School of Biological SciencesRoyal Holloway University of LondonSurreyUK
  5. 5.School of Biological and Chemical SciencesQueen Mary University of LondonLondonUK
  6. 6.Institute for BiologyMartin Luther-University Halle-WittenbergHalle (Saale)Germany
  7. 7.German Centre for Integrative Biodiversity Research Halle-Jena-Leipzig (iDiv)LeipzigGermany

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