Conservation Genetics

, Volume 18, Issue 3, pp 539–552 | Cite as

Phylogeography and historical demography of the orchid bee Euglossa iopoecila: signs of vicariant events associated to Quaternary climatic changes

  • Wilson Frantine-Silva
  • Douglas C. Giangarelli
  • Rafael E. S. Penha
  • Karen M. Suzuki
  • Enderlei Dec
  • Maria C. Gaglianone
  • Isabel Alves-dos-Santos
  • Silvia H. Sofia
Research Article


The aim of this study was to investigate whether Pleistocene climatic instability influenced the phylogeographic structure and historical demography of an endemic Atlantic Forest (AF) orchid bee, Euglossa iopoecila Dressler, which shows two main patterns of integument colors over of its geographical distribution. We based our analysis on the concatenated sequence of four mtDNA segments belonging to genes 16S (357 bp), Cytb (651 bp) and COI (1206 bp), totaling 2234 bp. Samples of E. iopoecila populations were collected in 14 AF remnants along its geographic distribution. Median-Joining haplotype networks, SAMOVA and BAPS results indicated three lineages (southern, central and northern clusters) for E. iopoecila, with two important phylogeographic ruptures. We found higher genetic diversity among samples collected in the central region of the AF, which coincides with predicted areas of climatic stability, according to recent AF stability–extinction model. The demographic analysis suggests that only the southern cluster had undergone recent population expansion, which probably started after the last glacial maximum (LGM). Our data suggest that the differentiation observed in the three mitochondrial lineages of E. iopoecila is the result of past disconnections and multiple extinction/recolonization events involving climate fluctuations. In terms of conservation, we would emphasize the importance of considering: (1) the region of the central clade as the location of the highest genetic diversity of mtDNA of E. iopoecila populations; (2) the philopatric behavior of females that tends to restrict mtDNA gene flow in particular, with direct implications for the conservation of the total genetic diversity in euglossine populations.


Euglossa iopoecila Euglossine Mitochondrial markers Genetic structure Orchid bee Bee conservation 



We would like to thank: a) CNPq and Fundação Araucária for financial support; b) CAPES for a scholarship awarded to Wilson Frantine-Silva and CNPq for the postdoctoral fellowship to Douglas C. Giangarelli; c) IBAMA/ICMBio, IAP and Instituto Florestal de São Paulo for the license for the collections; d) Reserva Natural Salto Morato (RNSM), Rebio União, Rebio Sooretama, Parque Nacional do Descobrimento, Parque Estadual Serra do Conduru, Estação Ecológica de Murici for the collecting permits; e) the staff of the State Parks of São Sebastião, Ilhabela, Ilha do Cardoso, Ubatuba (Picinguaba), RNSM and Parque Nacional do Superagui for providing infrastructure and their help in the field work; f) André Nemésio by donation of the specimens of E. iopoecila from sites BA3 and AL. Silvia H. Sofia, Maria C. Gaglianone and Isabel Alves-dos-Santos are research fellows from CNPq. We also would like to thank the reviewers for their suggestions, which substantially contributed to this paper improvement.

Supplementary material

10592_2016_905_MOESM1_ESM.jpg (277 kb)
Supplementary material 1 (JPEG 277 kb). Figure S1. a) Relationship between the genetic distances measured as F ST/(1 − F ST) and the logarithm of geographical distance (km); b) genetic distances (F ST) and geographic distance (km) between pairs of Euglossa iopoecila samples surveyed across the Brazilian Atlantic Forest. Minimum distance was around 2 km (SP3-SP4) and maximum distance was 2341 km (SC- AL)
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Supplementary material 2 (DOCX 14 kb)
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Supplementary material 3 (DOCX 22 kb)
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Supplementary material 4 (DOCX 56 kb)
10592_2016_905_MOESM5_ESM.docx (17 kb)
Supplementary material 5 (DOCX 15 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Wilson Frantine-Silva
    • 1
  • Douglas C. Giangarelli
    • 1
  • Rafael E. S. Penha
    • 2
  • Karen M. Suzuki
    • 1
  • Enderlei Dec
    • 3
  • Maria C. Gaglianone
    • 4
  • Isabel Alves-dos-Santos
    • 3
  • Silvia H. Sofia
    • 1
  1. 1.Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Departamento de Genética, Evolução e Bioagentes, Instituto de BiologiaUniversidade Estadual de CampinasCampinasBrazil
  3. 3.Departamento de Ecologia, Instituto de BiociênciasUniversidade de São PauloSão PauloBrazil
  4. 4.Laboratório de Ciências Ambientais, Programa de Pós-Graduação em Ecologia e Recursos NaturaisUniversidade Estadual do Norte FluminenseCampos dos GoytacazesBrazil

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