Conservation Genetics

, Volume 17, Issue 3, pp 615–630 | Cite as

Adaptive and neutral genetic differentiation among Scottish and endangered Irish red grouse (Lagopus lagopus scotica)

  • Yvonne Meyer-Lucht
  • Kevin P. Mulder
  • Marianne C. James
  • Barry J. McMahon
  • Kieran Buckley
  • Stuart B. Piertney
  • Jacob Höglund
Research Article

Abstract

Studying patterns of intra-specific genetic variation among populations allows for a better understanding of population structure and local adaptation. However, those patterns may differ according to the genetic markers applied, as neutral genetic markers reflect demographic processes and random genetic drift, whereas adaptive markers also carry the footprint of selection. In combination, neutral and adaptive genetic markers permit to assess the relative roles of drift and selection in shaping population structure. Among the best understood adaptive genetic loci are the genes of the major histocompatibility complex (MHC). We here study variation and differentiation at neutral SNP markers and MHC class II genes in red grouse (Lagopus lagopus scotica) from Ireland and Scotland. Irish red grouse populations are fragmented and drastically declining, but red grouse are abundant in Scotland. We find evidence for positive selection acting on the MHC genes and variation in MHC gene copy numbers among Irish individuals. Furthermore, there was significant population differentiation among red grouse from Ireland and Scotland at the neutral SNP markers (FST = 0.084) and the MHC-BLB genes (FST: BLB1 = 0.116, BLB2 = 0.090, BLB3 = 0.104). Differentiation at the MHC-BLB1 was significantly higher than at the neutral SNP markers, suggesting that selection plays an important role in shaping MHC variation, in addition to genetic drift. We speculate that the observed differentiation pattern might be due to local adaptation to different parasite regimes. These findings have strong conservation implications and we advise against the introduction of Scottish red grouse to supplement Irish populations.

Keywords

Major histocompatibility complex Single locus amplification MHC BLB exon 2 Galliformes Local adaptation Genetic drift 

Notes

Acknowledgements

We are very thankful to Ahmed Sayadi for helping with the resampling procedures, to Gunilla Engström for support in the genetic lab, and to Magnus Johansson for helpful discussions. Moreover, we thank the four anonymous reviewers for helpful and constructive advice on this manuscript. Irish feather samples were collected all over Ireland by volunteers from the Irish Grey Partridge Conservation Trust, the National Association of Regional Games Councils of Ireland, the Irish National Park and Wildlife Service, BirdWatch Ireland and red grouse enthusiasts from the Republic of Ireland. We thank staff at the NERC Biomolecular Analysis Facility at Sheffield for SNP genotyping. This study was funded by the Carl Tryggers foundation, the Erasmus Mundus master program in Evolutionary Biology (MEME), the Helge Ax:son Johnson foundation, the Native Species Conservation Committee of Dublin Zoo, Fota Wildlife Park, the UK Natural Environment Research Council and the Swedish Research Council.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10592_2016_810_MOESM1_ESM.pdf (573 kb)
Supplementary material 1 (PDF 573 kb)
10592_2016_810_MOESM2_ESM.pdf (451 kb)
Supplementary material 2 (PDF 451 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Yvonne Meyer-Lucht
    • 1
  • Kevin P. Mulder
    • 1
    • 2
  • Marianne C. James
    • 3
    • 4
  • Barry J. McMahon
    • 5
  • Kieran Buckley
    • 6
  • Stuart B. Piertney
    • 3
  • Jacob Höglund
    • 1
  1. 1.Department of Ecology and Genetics, Animal Ecology, Evolutionary Biology CenterUppsala UniversityUppsalaSweden
  2. 2.CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos da Universidade do PortoVairãoPortugal
  3. 3.Institute of Biological and Environmental SciencesUniversity of AberdeenAberdeenUK
  4. 4.Food Standards Agency in ScotlandAberdeenUK
  5. 5.UCD School of Agriculture & Food ScienceUniversity College DublinDublin 4Ireland
  6. 6.National Parks and Wildlife Service, Department of Arts, Heritage & the GaeltachtDublin 4Ireland

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