Past and present drivers of population structure in a small coastal fish, the European long snouted seahorse Hippocampus guttulatus
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The effective design of species conservation programs is reliant on information such as extant geographic distribution, taxon-specific life-history characteristics, and the relative influence of historic processes and contemporary environmental parameters in shaping population genetic diversity. Seahorses are weak swimmers and have a brooded young, limiting their dispersal potential. They live in sheltered locations, which are physically isolated from each other. Therefore panmixia across their geographic range is unlikely. Hippocampus guttulatus, a seahorse inhabiting European waters, has a geographic range spanning a number of contemporary oceanographic features that are proposed barriers to gene flow. Thus this fish is well-placed to test the contributions of environment and life-history factors in shaping population structuring. This study found that mitochondrial DNA and nuclear DNA (microsatellite) genotype data are concordant in suggesting that, like many other small fishes in European waters, H. guttulatus extant populations expanded from at least one southern European refugial population. Subsequent population differentiation of four geographic lineages reflects contemporary oceanographic barriers to gene flow. Demographic analyses suggest a northward, and long-term isolation between Black Sea and Mediterranean Sea populations. Moreover H. guttulatus contemporary population distribution and population structure are predominately explained by historic and oceanographic influences. These findings suggest that conservation of genetic diversity in H. guttulatus may be aided by a network of marine protected areas (MPAs), implemented to conserve coastal habitats, but the species’ unusual life history and gamete retaining behaviours should be considered as part of management decisions including MPA design and fisheries management plans.
KeywordsHippocampus guttulatus Conservation genetics Phylogeography Europe Seahorse
This is a contribution from Project Seahorse. We gratefully acknowledge France: C. L. Milinaire, P. Moriniere, S. Auffret, X. De Montandouin, P. Louisy, J-B Senegas, P. LeLong, Spain: J.A. Rodriguez, Scubadoo, A.Martinez de Murguia, B. Moya, Greece: F. Vilanikis, Y. Issaris, M. Salomidi, Tethys dive club, Thalassa, Portugal, Parque Natural da Ria Formosa, M. Gaspar, F. Gil, Italy: A. Tavaglini, S. Repetto, L. Castellano, Bulgaria: A. Seaman, T. Gallati, Gallati Divecenter, Volunteers: J. Marcus, V. Santos, D. Mason, M. Naud, T-T. Ang and S. DeAmicis for support with field work and providing samples. We also express thanks to J. Curtis and N. McKeown for enlightening discussion and technical assistance. Finally we wish to thank reviewers for their useful comments.
Conflict of interest
The authors declare that they have no conflict of interest.
This project was funded by Chocolaterie Guylian and a Natural Environment Research Council Industrial Case studentship (NER/S/C/2005/13461) to LCW.
- Boehm JT, Woodall LC, Teske PR, Lourie SA, Baldwin C, Waldman J, Hickerman M (2013) Marine dispersal and barriers drive Atlantic seahorse diversification. J Biogeogr 40:1839–1849Google Scholar
- Boisseau J (1967) Les régulations hormonales de l’incubation chez un vertèbre male: recherches sur la reproduction de l’hippocampe. PhD thesis, L’Université de Bordeaux, FranceGoogle Scholar
- Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 126.96.36.199). Universite de Lausanne, LausanneGoogle Scholar
- Green EP, Short FT (2003) World atlas of seagrasses. University of California Press, BerkeleyGoogle Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Lopez A, Vera M, Planas M, Bouza C (2015) Conservation genetics of threatened Hippocampus guttulatus in vulnerable habitats in NW Spain: temporal and spatial stability of wild populations with flexible polygamous mating system in captivity. PLoS One 10:e0117538CrossRefPubMedCentralPubMedGoogle Scholar
- Lourie SA, Foster SJ, Cooper EWT, Vincent ACJ (2004) A guide to the identification of seahorses, Washington D.C.Google Scholar
- Riccioni G, Landi M, Ferrara G, Milano I, Cariani A, Zane L, Sella M, Barbujan G, Tinti F (2010) Spatio-temporal population structuring and genetic diversity retention in depleted Atlantic Bluefin tuna of the Mediterranean Sea. Proc Natl Acad Sci USA 107:2102–2107CrossRefPubMedCentralPubMedGoogle Scholar
- Serra IA, Innocenti AM, Dimaida G, Calvo S, Migliaccio M, Zambianchi E, Pizzigalli C, Arnaud-Haond S, Duarte CM, Serrao EA, Procaccini G (2010) Genetic structure in the Mediterranean seagrass Posidonia oceanica: disentangling past vicariance events from contemporary patterns of gene flow. Mol Ecol 19:557–568CrossRefPubMedGoogle Scholar
- Sorokin YI (2002) The Black Sea: ecology and oceanography. Backhuis Publishing, LeidenGoogle Scholar
- Swofford DL (2003) PAUP*. Phylogenetic analysis using parsimony (* and other methods). Sinauer Associates, SunderlandGoogle Scholar
- Vandendriessche S, Messiaen M, Vincx M, Degraer S (2005) Juvenile Hippocampus guttulatus from a neuston tow at the French-Belgian border. Belg J Zool 135:101–102Google Scholar
- Woodall LC (2009) The population genetics and mating systems of European seahorses. PhD thesis, Royal Holloway-University of London, UKGoogle Scholar
- Woodall LC (2012) Hippocampus guttulatus. In: IUCN 2013. IUCN Red List of Threatened Species, Version 2013.1 http://www.iucnredlist.org. Assessed 24 Nov 2013