Conservation Genetics

, Volume 16, Issue 3, pp 687–702

Population genetics of Philotheca sporadica (Rutaceae) to advise an offset translocation program

  • Alison Shapcott
  • Robert W. Lamont
  • Katie M. O’Connor
  • Heather James
  • Gabriel C. Conroy
Research Article

Abstract

Philotheca sporadica (Rutaceae) is a vulnerable species restricted to a small geographic area, with very few populations protected in reserves. Environmental impact surveys identified some populations that will be impacted by gas pipeline construction. This study aimed to determine the genetic diversity of P. sporadica to advise an offset planting program. P. sporadica was found to have high population genetic diversity but all populations were genetically similar except two isolated genetically depauperate populations. Genetic diversity decreased with decreasing population size and increasing population isolation. Impacted populations were significant for the species viability, being among the largest and most genetically diverse, although a high percentage of populations were reproductively active and not inbred. A congeneric species P. difformis ssp. difformis that grows in the nearby vicinity was shown to be genetically distinct but some evidence of hybridisation was found suggesting that offset populations should not be located near known populations of this species to prevent further hybridisation.

Keywords

Endangered species Genetic diversity Gene flow Hybridisation Offsets Restoration Translocation 

Supplementary material

10592_2014_693_MOESM1_ESM.docx (39 kb)
Supplementary material 1 (DOCX 39 kb)

References

  1. Accad A, Neldner VJ, Wilson BA, Niehus RE (2013) Remnant Vegetation in Queensland. Analysis of remnant vegetation 1997–2011, including regional ecosystem information. Queensland Department of Science, Information Technology, Innovation and the Arts, BrisbaneGoogle Scholar
  2. Auld TD (2001) The ecology of the Rutaceae in the Sydney region of south-eastern Australia: poorly known ecology of a neglected family. Cunning 7:213–239Google Scholar
  3. Broadhurst LM, Coates DJ, Tan BH (1999) Genetic diversity in the monospecific Western Australian endemic, Geleznowia verrucosa Turcz. (Rutaceae). Hered 82:292–299CrossRefGoogle Scholar
  4. Brownlie H, Playford J, Wallace H, Shapcott A (2010) Population ecology and genetics of the vulnerable Acacia attenuata (Mimosaceae) and their significance for its conservation, recovery and translocation. Aust J Bot 57:675–687CrossRefGoogle Scholar
  5. Chung MY, Nason JD, Chung MG (2005) Patterns of hybridization and population genetic structure in the terrestrial orchids Liparis kumokiri and Liparis makinoana (Orchidaceae) in sympatric populations. Mol Ecol 14:4389–4402CrossRefPubMedGoogle Scholar
  6. Clarke K, Gorley R (2001) Plymouth routines in multivariate ecological research. PRIMER-E Ltd, PlymouthGoogle Scholar
  7. Coates D, Sokolowski R (1992) The mating system and patterns of genetic variation in Banksia cuneata A. S. George (Proteaceae). Hered 69:11–20CrossRefGoogle Scholar
  8. Coates DJ, Sampson JF, Yates CJ (2007) Plant mating systems and assessing population persistence in fragmented landscapes. Aust J Bot 55:239–249CrossRefGoogle Scholar
  9. Dwan BE, Trueman SJ (2014) Propagation methods for environmental off sett planting of the Kogan wax flower (Philotheca sporadica) (Rutaceae). J Environ Sci Tech 7:347–353CrossRefGoogle Scholar
  10. Earl DA, von Holdt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Cons Genet Res 4:359–361CrossRefGoogle Scholar
  11. Ellstrand NC (2014) Is gene flow the most important evolutionary force in plants? Am J Bot 101:737–753CrossRefPubMedGoogle Scholar
  12. Ellstrand NC, Elam DR (1993) Population genetic consequences of small population size: implications for plant conservation. Ann Rev Ecol Syst 24:217–242CrossRefGoogle Scholar
  13. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620CrossRefPubMedGoogle Scholar
  14. Excoffier L, Smouse PE, Quattro J (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction sites. Genetics 131:479–491PubMedCentralPubMedGoogle Scholar
  15. Falk D, Knapp E, Guerrant E (2001) An introduction to restoration genetics. EP Agency, Washington DCGoogle Scholar
  16. Falush D, Stephens M, Pritchard JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–1587PubMedCentralPubMedGoogle Scholar
  17. Falush D, Stephens M, Pritchard JK (2007) Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol Ecol Notes 7:574–578CrossRefPubMedCentralPubMedGoogle Scholar
  18. Fiedler PL, Laven RD (1996) Selecting reintroduction sites. In: Falk DA, Millar CI, Olwell M (eds) Restoring diversity; strategies for reintroductions of endangered plants. Island Press, Washington DC, pp 157–169Google Scholar
  19. Frankham R, Ballou LD, Bricoe D (2002) Introduction to conservation genetics. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  20. Frankham R, Ballou LD, Eldridge MDB, Lacy RC, Ralls K, Dudash MR, Fenster CB (2011) Predicting the probability of outbreeding depression. Cons Biol 25:465–475CrossRefGoogle Scholar
  21. Frankham R, Brook BW, Bradshaw CJA, Traill LW, Spielman D (2013) 50/500 rule and minimum viable populations: response to Jamieson and Allendorf. TREE 28:187–188PubMedGoogle Scholar
  22. Ghazoul J (2005) Pollen and seed dispersal among dispersed plants. Biol Rev 80:413–443CrossRefPubMedGoogle Scholar
  23. Godefroid S, Piazza C, Rossi G, Buord S, Stevens A-D, Aguraiuja R, Cowell C, Weekley CW, Vogg G, Iriondo JM, Johnson I, Dixon B, Gordon D, Magnanon S, Valentin B, Bjureke K, Koopman R, Vicens M, Virevaire M, Vanderborght T (2011) How successful are plant species reintroductions? Biol Cons 144:672–682CrossRefGoogle Scholar
  24. Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (v.2.9.3). http://www2.unil.ch/popgen/softwares/fstat.htm
  25. Halbur MM, Sloop CM, Zanis MJ, Emery NC (2014) Population biology of mitigation: impacts of habitat creation on an engangered plant species. Cons Genet 15:679–695CrossRefGoogle Scholar
  26. Halford D (1995) Eriostemon sporadicus Bayley (Rutaceae). A conservation statement—a report submitted to the Australian Nature Conservation Agency Endangered Species Program Project No. 482. Australian Nature Conservation Agency, CanberraGoogle Scholar
  27. Hamrick JL, Murawski DA, Nason JD (1993) The influence of seed dispersal mechanisms on the genetic structure of tropical tree populations. Vegetation 108:281–297Google Scholar
  28. Hensen I, Oberprieler C (2005) Effects of population size on genetic diversity and seed production in the rare Dictamnus albus (Rutaceae) in Germany. Cons Genet 6:63–73CrossRefGoogle Scholar
  29. Jones TA, Monaco TA (2007) A restoration practitioner’s guide to the restoration gene pool concept. Ecol Restor 25:12–19CrossRefGoogle Scholar
  30. Jump AS, Peñuelas J (2005) Running to stand still: adaptation and the response of plants to rapid climate change. Ecol Lett 8:1010–1020CrossRefGoogle Scholar
  31. Keller M, Kollmann J, Edwards PJ (2000) Genetic introgression from distant provenances reduces fitness in local weed populations. J Appl Ecol 37:647–659CrossRefGoogle Scholar
  32. Krauss SL, Hermanutz L, Hopper SD, Coates DJ (2007) Population-size effects on seeds and seedlings from fragmented eucalypt populations: implications for seed sourcing for ecological restoration. Aust J Bot 55:390–399CrossRefGoogle Scholar
  33. Lamont RW, Stokoe RL, Shapcott A (2012) Ecological genetics of the wind-pollinated, tetraploid, Allocasuarina emuina L. Johnson (Casuarinaceae) from southeast Queensland reveals montane refugia for coastal heath during the last interglacial. Aust J Bot 60:718–734CrossRefGoogle Scholar
  34. Leimu R, Mutikainen PIA, Koricheva J, Fischer M (2006) How general are positive relationships between plant population size, fitness and genetic variation? J Ecol 94:942–952CrossRefGoogle Scholar
  35. Levin DA, Francisco-Ortega J, Jansen RK (1996) Hybridization and the extinction of rare species. Cons Biol 10:10–16CrossRefGoogle Scholar
  36. Lopez S, Rousset F, Shaw F, Shaw R, Ronce O (2009) Joint effects of inbreeding and local adaptation on the evolution of genetic load after fragmentation. Cons Biol 23:1618–1622CrossRefGoogle Scholar
  37. Lynch AJ, Vaillancourt RE (1995) Genetic diversity in the endangered Phebalium daviesii (Rutaceae) compared to that in two widespread congeners. Aust J Bot 43:181–191CrossRefGoogle Scholar
  38. McKay JK, Christian CE, Harrison S, Rice KJ (2005) How local is local?—a review of practical and conceptual issues in the genetics of restoration. Rest Ecol 13:432–440CrossRefGoogle Scholar
  39. Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinfo 26:403–404CrossRefGoogle Scholar
  40. Menz MHM, Phillips RD, Winfree R, Kremen C, Aizen MA, Johnson SD, Dixon KW (2011) Reconnecting plants and pollinators: challenges in the restoration of pollination mutualisms. Trends Plant Sci 16:1360–1385CrossRefGoogle Scholar
  41. Moilanen A, van Teeffelen AJA, Ben-Haim Y, Ferrier S (2009) How much compensation is enough? A framework for incorporating uncertainty and time discounting when calculating offset ratios for impacted habitat. Restor Ecol 17:470–478CrossRefGoogle Scholar
  42. Pasquet RS, Peltier A, Hufford MB, Oudin E, JSaulnier J, Le´naic P, Knudsen JT, Herren HR, Gepts P (2008) Long-distance pollen flow assessment through evaluation of pollinator foraging range suggests transgene escape distances. PNAS 105:13456–13461CrossRefPubMedCentralPubMedGoogle Scholar
  43. Peakall R, Smouse PE (2006) Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  44. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959PubMedCentralPubMedGoogle Scholar
  45. Ren H, Zhang Q, Wang Z, Guo Q, Wang J, Liu N, Liang K (2010) Conservation and possible reintroduction of an endangered plant based on an analysis of community ecology: a case study of Primulina tabacum Hance in China. Plant Species Biol 25:43–50CrossRefGoogle Scholar
  46. Rhymer JM, Simberloff D (1996) Extinction by hybridization and introgression. Ann Rev Ecol Syst 27:83–109CrossRefGoogle Scholar
  47. Rieseberg LH (1997) Hybrid origins of plant species. Ann Rev Ecol Syst 28:359–390CrossRefGoogle Scholar
  48. Selbo SM, Snow AA (2005) Flowering phenology and genetic similarity among local and recently introduced populations of Andropogon gerardii in Ohio. Restor Ecol 13:441–447CrossRefGoogle Scholar
  49. Shapcott A, Powell M (2011) Demographic structure, genetic diversity and habitat distribution of the endangered, Australian rainforest tree Macadamia jansenii help facilitate a reintroduction program. Aust J Bot 59:215–225CrossRefGoogle Scholar
  50. Shapcott A, Lamont RW, Thomson A (2005) How do rare Boronia species differ from their more widespread congeners? Aust J Bot 53:171–183CrossRefGoogle Scholar
  51. Shapcott A, Olsen M, Lamont RW (2009) The importance of genetic considerations for planning translocations of the rare coastal heath species Boronia rivularis (Rutaceae) in Queensland. Ecol Restor 27:47–57CrossRefGoogle Scholar
  52. Simmons CL, Auld TD, Hutton I, Baker WJ, Shapcott A (2012) Will climate change, genetic and demographic variation or rat predation pose the greatest risk for persistence of an altitudinally distributed island endemic? Biol 1:736–765CrossRefGoogle Scholar
  53. Suding KN (2011) Toward an era of restoration in ecology: successes, failures, and opportunities ahead. Ann Rev Ecol Evol Syst 42:465–487CrossRefGoogle Scholar
  54. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICROCHECKER (Version 2.2.3): software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  55. Weeks AR, Sgro CM, Young AG, Frankham R, Mitchell MJ, Miller KA, Byrne M, Coates DJ, Eldridge MDB, Sunnucks P, Breed MF, James EA, Hoffman AA (2011) Assessing the benefits and risks of translocations in changing environments: a genetic perspective. Evol Appl 4:705–724CrossRefGoogle Scholar
  56. Willi Y, van Buskirk J, Hoffmann AA (2006) Limits to the adaptive potential of small populations. Ann Rev Ecol Evol Syst 37:433–478CrossRefGoogle Scholar
  57. Yang Y, Pan Y, Gang Y, Fan M (2010) Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting. Genet Resour Crop Evol 57:1239–1248CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Alison Shapcott
    • 1
  • Robert W. Lamont
    • 1
  • Katie M. O’Connor
    • 1
  • Heather James
    • 1
  • Gabriel C. Conroy
    • 1
  1. 1.Faculty of Science, Health, Education and Engineering, Genecology Research CentreUniversity of the Sunshine CoastMaroochydore DCAustralia

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