Conservation Genetics

, Volume 16, Issue 3, pp 595–611 | Cite as

Toll-like receptor diversity in 10 threatened bird species: relationship with microsatellite heterozygosity

  • Catherine E. Grueber
  • Gabrielle J. Knafler
  • Tania M. King
  • Alistair M. Senior
  • Stefanie Grosser
  • Bruce Robertson
  • Kerry A. Weston
  • Patricia Brekke
  • Christian L. W. Harris
  • Ian G. Jamieson
Research Article

Abstract

Measuring individual-level heterozygosity in threatened species is one approach to understanding and mitigating losses of genetic diversity and the role of inbreeding depression in those populations. In many conservation contexts, this goal is approached by assaying levels of microsatellite diversity, and inference is often extended to functional genomic regions. Our study quantifies diversity of innate immunity toll-like receptor (TLR) genes in 10 threatened New Zealand birds across four avian orders, with an average of 20.1 individuals and 6.2 TLR loci (sequences averaging 850 bp in length) per species. We provide detailed TLR diversity statistics for these 10 species, which showed more evidence for genetic drift than balancing selection at TLR loci, with two possible exceptions (TLR1LA for hihi and TLR5 for kokako). Our observations also support a possible gene-duplication of TLR7 in rock wren, indicating that a TLR7 duplication previously observed in other passerines may have occurred early in the divergence of this order. In addition to these analyses of population-level TLR sequence diversity, we used an average of 14.6 polymorphic microsatellite loci per species to study, for the first time, the relationship between microsatellite internal relatedness (a measure of individual homozygosity) and TLR heterozygosity. There was no relationship between microsatellite and TLR heterozygosity of individuals within species, suggesting that the predictive power of microsatellites to evaluate functional diversity is poor, and highlighting the value of adding data from putatively functional genomic regions, such as TLRs, in the study of genetic diversity of threatened species. Overall this study provides valuable data for comparison with more widespread species, and facilitates research into the importance of TLR diversity in natural populations of conservation concern.

Keywords

Apterygiformes Conservation Gruiformes Immunity genes Inbreeding Passeriformes Psittaciformes 

Notes

Acknowledgments

We are grateful to those who generously provided samples and support for this study: Hugh Robertson, Oliver Overdyck, Tertia Thurley (New Zealand Department of Conservation); Kevin Parker (Massey University), John Ewen (Zoological Society of London) and Bethany Jackson (Auckland Zoo). We also thank Fiona Robertson for laboratory assistance and Ken Miller for preparation of Fig. 1. We are grateful for the continuing support of the New Zealand Department of Conservation, and in particular those Species Recovery Groups included in this study. This research was supported by the Allan Wilson Centre for Molecular Ecology and Evolution, the Marsden Fund, Landcare Research, University of Otago, a Royal Society Grant to PB and grants from Brian Mason Scientific and Technical Trust, Mohua Charitable Trust and JS Watson Conservation Trust to KW. CEG is currently supported by San Diego Zoo Global.

Supplementary material

10592_2014_685_MOESM1_ESM.docx (254 kb)
Supplementary material 1 (DOCX 255 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Catherine E. Grueber
    • 1
    • 5
  • Gabrielle J. Knafler
    • 1
  • Tania M. King
    • 1
  • Alistair M. Senior
    • 2
  • Stefanie Grosser
    • 1
  • Bruce Robertson
    • 1
  • Kerry A. Weston
    • 1
  • Patricia Brekke
    • 3
  • Christian L. W. Harris
    • 4
  • Ian G. Jamieson
    • 1
  1. 1.Allan Wilson Centre for Molecular Ecology and Evolution, Department of ZoologyUniversity of OtagoDunedinNew Zealand
  2. 2.Charles Perkins Centre and School of Biological SciencesUniversity of SydneySydneyAustralia
  3. 3.Institute of ZoologyZoological Society of LondonLondonUK
  4. 4.Department of BiochemistryUniversity of OtagoDunedinNew Zealand
  5. 5.Faculty of Veterinary ScienceUniversity of SydneySydneyAustralia

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