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Conservation Genetics

, Volume 16, Issue 3, pp 523–533 | Cite as

Multivariate analysis of polyploid data reveals the role of railways in the spread of the invasive South African Ragwort (Senecio inaequidens)

  • Élodie Blanchet
  • Caterina PenoneEmail author
  • Noëlie Maurel
  • Claire Billot
  • Ronan Rivallan
  • Ange-Marie Risterucci
  • Sandrine Maurice
  • Fabienne Justy
  • Nathalie Machon
  • Florence Noël
Research Article

Abstract

Genetic studies constitute a powerful approach to study the introduction and expansion of invasive species. Senecio inaequidens DC is one of the Europe’s fastest plant invaders and is suspected to use railway corridors to expand. In France, the species has first been observed in the city of Paris in the late 1990’s and today it is present throughout the region. In order to assess the role of railways in S. inaequidens invasion, we used innovative multivariate analyses to study population genetic diversity of this allotetraploid species. We analyzed the genetic diversity at 11 microsatellite markers of a total of 450 individuals sampled at 15 locations along railways connecting the city of Paris to the surrounding suburban/rural areas. Geographical distances among locations ranged from 1 to 83 km with four locations within Paris at the departure train stations of the national/international train lines studied. To our knowledge, studies on invasive allotetraploid plants where data were kept in the tetraploid format are still scarce. Our analyses revealed substantial genetic diversity and clear genetic differentiation among some populations, with a genetic gradient detected along one railway line. Results also showed no genetic bottleneck between Parisian populations and the historically introduced population located in South of France. Our study thus revealed the role of railways as a corridor among S. inaequidens populations within central Paris. However, connecting networks appeared more complex in the suburban and rural areas suggesting other potential vectors.

Keywords

Multivariate analyses Corridors Genetic diversity Isolation by distance SSR markers Tetraploidy 

Notes

Acknowledgments

This work was supported by the Réseau de Recherche sur le Développement Soutenable (R2DS); and the Société Nationale des Chemins de fer Français (SNCF). The authors would like to thank the three anonymous reviewers and the editor A. Jump for their helpful comments on a previous version of the manuscript.

Supplementary material

10592_2014_676_MOESM1_ESM.doc (899 kb)
ESM_A1: Map of site locationsESM_A2: PCR and genotyping conditionsESM_A3: Genetic differentiation between DAPC clustersESM_A4: Genetic differentiation parameters between all populationsESM_A5: Bayesian analysesESM_A6: Railway bridges along North, East and South linesSupplementary material 1 (DOC 899 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Élodie Blanchet
    • 1
  • Caterina Penone
    • 1
    • 2
    Email author
  • Noëlie Maurel
    • 1
    • 3
  • Claire Billot
    • 4
  • Ronan Rivallan
    • 4
  • Ange-Marie Risterucci
    • 4
  • Sandrine Maurice
    • 5
  • Fabienne Justy
    • 5
  • Nathalie Machon
    • 1
  • Florence Noël
    • 1
  1. 1.Centre d’Écologie et des Sciences de la Conservation (CESCO) UMR 7204, Muséum National d’Histoire Naturelle (MNHN)CNRS & UPMCParisFrance
  2. 2.Departamento de EcologiaUniversidade Federal do Rio Grande do NorteNatalBrazil
  3. 3.Ecology, Department of BiologyUniversity of KonstanzConstanceGermany
  4. 4.CIRADUMR AGAPMontpellierFrance
  5. 5.Institut des Sciences de l’Évolution (ISEM), UMR 5554Université Montpellier IIMontpellier Cedex 05France

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