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Conservation Genetics

, Volume 16, Issue 2, pp 431–441 | Cite as

Eco-geographic units, population hierarchy, and a two-level conservation strategy with reference to a critically endangered salmonid, Sakhalin taimen Parahucho perryi

  • Lev A. Zhivotovsky
  • Andrey A. Yurchenko
  • Vitaly D. Nikitin
  • Sergei N. Safronov
  • Marina V. Shitova
  • Sergei F. Zolotukhin
  • Sergei S. Makeev
  • Steven Weiss
  • Peter S. Rand
  • Anatoly Yu. Semenchenko
Research Article

Abstract

Hierarchical population structure can result from range-wide geographic subdivision under conditions of environmental heterogeneity and weak gene flow. While a lower level of structure can be formed by local populations within eco-geographic regions, an upper level can be characterized by variation between populations from different regions, and thus, be represented by evolutionarily significant units (ESUs) defined by environmental, ecological and genetic variation. Selection of ESUs may depend on the sequence of using these three sources of variation. We propose to determine ESUs by first using non-genetic, ecological and geographical gradients for defining preliminary population groups (eco-geographic units, EGUs) and then testing whether the boundaries of these units are genetically coherent and thus represent ESUs or warrant their further modification. We evaluate this approach using Sakhalin taimen, an East Asian endangered endemic fish. Forty-one samples (473 fish) were drawn from thirty populations across the species range and genotyped at microsatellite DNA markers. We assign the populations into ESUs based on geographic and life history criteria and subsequent application of genetic diversity analyses. The ESUs appeared to be greatly diverged genetically. Within ESUs, local populations are genetically differentiated, have low effective sizes, show signatures of demographic decline and extremely restricted gene flow. Conservation plans aimed to restore or maintain a specific threatened population should take into account such hierarchical structure, and in particular be based on the genetic resources drawn from each population or using ecologically and genetically similar populations from the same ESU as donors for restoration of the population.

Keywords

Semi-anadromous salmonid fish Genetic divergence Reproductive isolation Restricted gene flow Small population and sample size Landscape genetics 

Notes

Acknowledgments

We are thankful to the Agency ‘Rosprirodnadzor’ for permissions to obtain genetic samples from Sakhalin taimen populations and to S. Didenko for setting up of field expeditions. We are indebted to D. Didenko (travel agency “Ostrov”) for his great help in sampling, as well as other samplers listed in Table A1. Field expeditions and genetic work were supported in part by Grants to L.Zh. from RAS program “Living Nature– Genofonds”, and from “Sakhalin Salmon Initiatives”; all participants were also supported by funds of their institutions, and P.R. by a Grant from National Geographic Society. We are grateful to Dr. Robin Waples and an anonymous reviewer for their valuable comments on the manuscript.

Supplementary material

10592_2014_670_MOESM1_ESM.docx (163 kb)
Supplementary material 1 (DOCX 162 kb)
10592_2014_670_MOESM2_ESM.docx (219 kb)
Supplementary material 2 (DOCX 219 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Lev A. Zhivotovsky
    • 1
  • Andrey A. Yurchenko
    • 1
  • Vitaly D. Nikitin
    • 2
  • Sergei N. Safronov
    • 2
  • Marina V. Shitova
    • 1
  • Sergei F. Zolotukhin
    • 3
  • Sergei S. Makeev
    • 4
  • Steven Weiss
    • 5
  • Peter S. Rand
    • 6
  • Anatoly Yu. Semenchenko
    • 7
    • 8
  1. 1.Institute of General GeneticsRussian Academy of SciencesMoscowRussia
  2. 2.Sakhalin Research Institute of Fisheries and OceanographyYuzhno-SakhalinskRussia
  3. 3.Khabarovsk Branch of Pacific Research Fisheries CentreKhabarovskRussia
  4. 4.FGUP “Sakhalinrybvod”Yuzhno-SakhalinskRussia
  5. 5.Institute of ZoologyGrazAustria
  6. 6.Wild Salmon CenterPortlandUSA
  7. 7.ANO “Sakhalin Salmon Initiatives”Yuzhno-SakhalinskRussia
  8. 8.Scientific and Educational Complex “Primorsky Aquarium”Far Eastern Branch of Russian Academy of SciencesVladivostokRussia

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