Conservation genetics of an endemic from the Mediterranean Basin: high genetic differentiation but no genetic diversity loss from the last populations of the Sicilian Grape Hyacinth Leopoldia gussonei
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The Mediterranean Basin is a biodiversity hotspot, housing >11.000 narrowly endemic plant species, many of which are declining due to mass tourism and agricultural intensification. To investigate the genetic resource impacts of ongoing habitat loss and degradation, we characterized the genetic variation in the last known populations of Leopoldia gussonei, a self-compatible endangered Sicilian Grape Hyacinth numbering less than 3,000 remaining individuals, using AFLP. Results demonstrated significant genome-wide genetic differentiation among all extant populations (ΦST = 0.05–0.56), and genetic clustering according to geographic location. Gene diversity was fairly constant across population (mean HE = 0.13) and was neither affected by current population size nor by spatial isolation. Vegetation analysis showed the presence of known invasive weeds in a quarter of the populations, but we found no relation between genetic diversity and plant community composition. The marked genetic differences among populations and the profusion of rare and private alleles indicate that any further population loss will lead to significant losses of genetic diversity. Conservation efforts should therefore focus on the preservation of all sites where L. gussonei still occurs, yet the deliberate introduction of diverse material into the smallest populations seems unneeded as clonality likely mitigated genetic drift effects thus far. More generally, our findings support the view that endemic plant species with a narrow ecological amplitude, as many specialists in Mediterranean coastal ecosystems, are highly genetically differentiated and that conservation of their genetic diversity requires preservation of most, if not all of their extant populations.
KeywordsMediterranean Basin AFLP Asparagaceae Habitat loss Habitat degradation Narrow endemics
KV holds a postdoctoral fellowship of the Research Foundation—Flanders (FWO).
- Albo G (1919) La vita delle piante vascolari nella Sicilia meridionale-orientale. Parte II, FloraGoogle Scholar
- Brullo S, Marcenò C (1974) Vulpio-Leopoldietum gussonei ass. nov. dell’Alkanneto-Malcolmion nella Sicilia meridionale. Notiziario Fitosociologico 8:75–85Google Scholar
- Brullo C, Giusso del Galdo G, Marcenò C, Minissale P, Sciandrello S (2011) Leopoldia gussonei. In: IUCN 2012. IUCN Red list of threatened species. Version 2012.1. www.iucnredlist.org. Downloaded on 24 June 2012
- Cundari R, Fontana F, Giardina G, Longhitano N (2003) La flora della Riserva N.O. “Pino d’Aleppo” (Sicilia Meridionale-Orientale). In: Italian Botanical Society (ed.), 98° Congresso S.B.I. Riassunti, 229, CataniaGoogle Scholar
- Dallman PF (1998) Plant life in the World’s Mediterranean climates. California native plant society. University of California Press, BerkeleyGoogle Scholar
- Greuter W (1991) Botanical diversity, endemism, rarity, and extinction in the Mediterranean area: an analysis based on the published volumes of med-checklist. Bot Chron (Patras) 10:63–79Google Scholar
- USDA, NRCS (2010) The PLANTS database. National Plant Data Center, Baton RougeGoogle Scholar
- Vandepitte K, Gristina A, Meekers T, de Hert K, Roldan-Ruiz I, Honnay O (2012) Recolonization after habitat restoration leads to decreased genetic variation in populations of a terrestrial orchid. Mol Ecol (in press). doi: 10.1111/j.1365-294X.2012.05698.x
- Vekemans X (2002) AFLPsurv version 1.0: a program for genetic diversity analysis with AFLP (and RAPD) population data. Laboratoire de Génétique et Ecologie végétales, Université Libre de BruxellesGoogle Scholar
- Waples RS (1995) Evolutionarily significant units and the conservation of biological diversity under the Endangered Species Act. Am Fish Society Symp 17:8–27Google Scholar
- Wright S (1938) Size of population and breeding structure in relation to evolution. Science 87:430–431Google Scholar