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Conservation Genetics

, Volume 13, Issue 1, pp 223–234 | Cite as

Microsatellite evidence for high clonality and limited genetic diversity in Ziziphus celata (Rhamnaceae), an endangered, self-incompatible shrub endemic to the Lake Wales Ridge, Florida, USA

  • Matthew A. GitzendannerEmail author
  • Carl W. Weekley
  • Charlotte C. Germain-Aubrey
  • Douglas E. Soltis
  • Pamela S. Soltis
Research Article

Abstract

Genetic data are often crucial for designing management strategies for rare and endangered species. Ziziphus celata is an endangered sandhill shrub endemic to the Lake Wales Ridge of central Florida. This self-incompatible clonal species is known from only 14 wild populations, most of which are small (under 100 plants). Focusing on the five populations discovered in 2007, we evaluate the level of genetic diversity and identify clonal lineages within the wild populations of the species with a set of microsatellite loci. To account for somatic mutations and genotyping errors, we identified clonal lineages using a threshold cutoff for pair-wise genetic distances among samples. The microsatellites had up to 18 alleles/locus, and, consistent with outcrossing, samples were highly heterozygous (average population level H o  = 0.69). Most populations of Z. celata consist of a single clone, and the most diverse population has only 10 clones. Overall Z. celata comprises 41 multi-locus genotypes, and 30 clonal lineages. With nearly 1,000 recorded plants (595 genotyped) and only 30 clonal lineages, Ziziphus celata is highly clonal: clonal richness, R = 0.049. The pair-wise distance method facilitates identification of clonal lineages, avoiding overestimation of clonal diversity. In most cases, the samples that grouped into a lineage were one to four plants differing from a surrounding genotype by a single microsatellite repeat insertion/deletion mutation, consistent with these having arisen via somatic mutations. Our data will enable managers to incorporate extant diversity from wild populations into ex situ collections. Additionally, our research demonstrates the utility of microsatellites for conservation of imperiled species, identifying genotypes of high priority for preservation.

Keywords

Clonality Conservation genetics Genetic diversity Lake Wales Ridge, Florida Rare species conservation 

Notes

Acknowledgments

The authors thank S. Anak and P. Soria for assistance in the lab; funding was provided by the US Fish and Wildlife Service, the Plant Conservation Program of the Florida Division of Forestry, and Archbold Biological Station. We thank E.S. Menges and three anonymous reviewers for helpful comments on the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Matthew A. Gitzendanner
    • 1
    • 2
    Email author
  • Carl W. Weekley
    • 3
  • Charlotte C. Germain-Aubrey
    • 1
  • Douglas E. Soltis
    • 1
  • Pamela S. Soltis
    • 2
  1. 1.Department of BiologyUniversity of FloridaGainesvilleUSA
  2. 2.Florida Museum of Natural HistoryUniversity of FloridaGainesvilleUSA
  3. 3.Archbold Biological StationLake PlacidUSA

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