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Conservation Genetics

, Volume 11, Issue 2, pp 547–556 | Cite as

The genetic legacy of extirpation and re-colonization in Vancouver Island wolves

  • Violeta Muñoz-FuentesEmail author
  • Chris T. Darimont
  • Paul C. Paquet
  • Jennifer A. Leonard
Research Article

Abstract

Hybridization between wild and domestic species is of conservation concern because it can result in the loss of adaptations and/or disappearance of a distinct taxon. Wolves from Vancouver Island, British Columbia (Canada), have been subject to several eradication campaigns during the twentieth century and were considered virtually extirpated between 1950 and 1970. In this study, we use control region mitochondrial DNA sequences and 13 autosomal microsatellite loci to characterize Vancouver Island wolves as well as dogs from British Columbia. We observe a turnover in the haplotypes of wolves sampled before and after the 1950–1970 period, when there was no permanent wolf population on the island, supporting the probable local extinction of wolves on Vancouver Island during this time, followed by re-colonization of the island by wolves from mainland British Columbia. In addition, we report the presence of a domestic dog mtDNA haplotype in three individuals eliminated in 1986 that were morphologically identified as wolves. Here we show that Vancouver Island wolves were also identified as wolves based on autosomal microsatellite data. We attribute the hybridization event to the episodically small size of this population during the re-colonization event. Our results demonstrate that at least one female hybrid offspring, resulting from a cross of a male wolf and a female dog or a female hybrid pet with dog mtDNA, successfully introgressed into the wolf population. No dog mtDNA has been previously reported in a population of wild wolves. Genetic data show that Vancouver Island wolves are distinct from dogs and thus should be recognized as a population of wild wolves. We suggest that the introgression took place due to the Allee effect, specifically a lack of mates when population size was low. Our findings exemplify how small populations are at risk of hybridization.

Keywords

Allee effect Canada Canis Domestic dog Historical DNA Hybridization Introgression Microsatellites mtDNA Museum specimens Wolf 

Notes

Acknowledgments

We thank Heather Bryan for sharing dog samples with us and to Helen Schwantje of the British Columbia Ministry of Environment for providing contemporary wolf samples. We thank Rex Kenner of the University of British Columbia Cowan Vertebrate Museum and Jim Cosgrove from the Royal British Columbia Museum for access to samples in collections. We are also thankful to Carles Vilà and Robert K. Wayne for a critical reading of the manuscript. Funding was provided by the European Union Marie Curie Fellowship programme, the National Science and Engineering Research Council of Canada Postdoctoral Fellowship programme, the National Science Foundation, USA (OPP-0352634), the Spanish Ministry of Education, the Swedish Research Council, Raincoast Conservation Foundation, Wilburforce Foundation and World Wildlife Fund—Canada. The Center for Conservation & Evolutionary Genetics, National Zoological Park, Smithsonian Institution, USA, provided logistical support.

Supplementary material

10592_2009_9974_MOESM1_ESM.doc (272 kb)
(DOC 273 kb)

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Violeta Muñoz-Fuentes
    • 1
    Email author
  • Chris T. Darimont
    • 2
    • 3
  • Paul C. Paquet
    • 3
    • 4
  • Jennifer A. Leonard
    • 1
    • 5
    • 6
  1. 1.Department of Evolutionary BiologyUppsala UniversityUppsalaSweden
  2. 2.Environmental Studies DepartmentUniversity of CaliforniaSanta CruzUSA
  3. 3.Raincoast Conservation FoundationDenny IslandCanada
  4. 4.Faculty of Environmental DesignUniversity of CalgaryCalgaryCanada
  5. 5.Center for Conservation and Evolutionary GeneticsNational Zoological Park, Smithsonian InstitutionWashingtonUSA
  6. 6.Estación Biológica de Doñana-CSICSevillaSpain

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