Conservation Genetics

, Volume 11, Issue 4, pp 1453–1466 | Cite as

Multilocus phylogenetic and coalescent analyses identify two cryptic species in the Italian bianchetto truffle, Tuber borchii Vittad.

  • Enrico Bonuso
  • Alessandra Zambonelli
  • Sarah E. Bergemann
  • Mirco Iotti
  • Matteo Garbelotto
Research Article


Tuber borchii (Ascomycota, Pezizales) is a highly valued truffle sold in local markets in Italy. Despite its economic importance, knowledge on its distribution and genetic structure is scarce. The objective of this work was to investigate the factors shaping the genetic structure of T. borchii using 61 representative specimens with a broad distribution throughout Italy. In spite of the lack of morphological differences, phylogenetic and coalescent-based analyses using four loci identified two genetically isolated groups sympatrically distributed. The low levels of divergence between the two clades may be the result of recent range expansions from geographically distinct refugia, potentially mediated by reforestation using coniferous species that are common ectomycorrhizal symbionts for both groups.


Cryptic species Genetic isolation Genetic subdivision 



We would like to thank Dr. Enrico Lancellotti for his assistance in statistical analyses of morphological data.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Enrico Bonuso
    • 1
  • Alessandra Zambonelli
    • 1
  • Sarah E. Bergemann
    • 2
  • Mirco Iotti
    • 1
  • Matteo Garbelotto
    • 3
  1. 1.Dipartimento di Protezione e Valorizzazione Agroalimentare (DIPROVAL)University of BolognaBolognaItaly
  2. 2.Biology DepartmentMiddle Tennessee State UniversityMurfreesboroUSA
  3. 3.Department of Environmental Science, Policy, and Management, Ecosystem Sciences DivisionUniversity of California BerkeleyBerkeleyUSA

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