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Conservation Genetics

, Volume 11, Issue 3, pp 1185–1187 | Cite as

A sex determination protocol for the Iberian desman (Galemys pyrenaicus) based on a three primer amplification of DBX and DBY fragments with non-invasive samples

  • Oriol VidalEmail author
  • Alexandra Pérez-Serra
  • Carles Pla
Technical Note

Abstract

We have sequenced partial fragments of DBX and DBY genes of the endangered Iberian desman (Galemys pyrenaicus). The sequences were used to design a sex determination protocol for non-invasive samples based on a PCR reaction, using only three primers. This protocol allows the simultaneous amplification of two fragments, one corresponding to the DBX gene and the other to the DBY gene, both differing in size. To increase sensitivity on the detection of positive amplifications and on the determination of fragment size we use a fluorescently labelled primer. The protocol has been tested in DNA samples from hair and stool, revealing major difficulties in sexing faecal samples, but unambiguous sexing of hair samples.

Keywords

Iberian desman Non-invasive samples Sexing DBY DBX 

Notes

Acknowledgments

The authors thank Francesca Rivas for her technical assistance and Ángel Fernández for providing samples. Work funded by the Spanish Ministry of Education and Science (CGL2004-04368/BOS).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Oriol Vidal
    • 1
    Email author
  • Alexandra Pérez-Serra
    • 1
  • Carles Pla
    • 1
  1. 1.Laboratori d’Ictiologia Genètica, Departament de BiologiaUniversitat de GironaGironaSpain

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