Anonymous nuclear markers for cetacean species
- First Online:
- 239 Downloads
Here we report the development and characterization of 17 anonymous nuclear markers for cetacean species. These markers were isolated from a genomic library built from a common dolphin (genus Delphinus), and tested across several families within Cetacea. An average of 1 SNP per 272 bp was found in 10 anonymous markers screened for polymorphism within the genus Delphinus (total of 6,537 bp sequenced). These markers represent a significant addition to the set of tools used in genetic studies of cetaceans where population and species boundaries have to be inferred in order to implement proper conservation strategies.
KeywordsSNPs Anonymous loci Common dolphins Delphinids
- Caballero S, Jackson J, Mignucci-Giannoni AA, Barrios-Garrido H, Beltran-Pedreros S, Montiel-Villalobos MG, Robertson KM, Baker CS (2008) Molecular systematics of South American dolphins Sotalia: sister taxa determination and phylogenetic relationships, with insights into a multi-locus phylogeny of the Delphinidae. Mol Phylogenet Evol 46:252–268. doi:10.1016/j.ympev.2007.10.015 CrossRefPubMedGoogle Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on WWW for general users and for biologist programmars. In: Misener S, Krawetz S (eds) Bioinformatics methods protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386Google Scholar