Successful analysis of AFLPs from non-lethally sampled wing tissues in butterflies
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- Keyghobadi, N., Crawford, L.A. & Maxwell, S.A. Conserv Genet (2009) 10: 2021. doi:10.1007/s10592-009-9884-2
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We demonstrate the successful generation and analysis of amplified fragment length polymorphism (AFLP) profiles from small samples of wing tissue in two butterfly species. With slight modifications of commercial DNA extraction and AFLP kit protocols, we produced highly repeatable AFLP profiles from these non-destructive tissue samples. Error rates were comparable to those previously reported for AFLPs generated from lethally obtained thoracic tissue. Furthermore, AFLP profiles obtained from thoracic and wing tissues of the same individuals were identical. Our results indicate that AFLP analysis is a viable method of obtaining genetic data from threatened populations of butterflies, and potentially other insects, using small, non-destructive tissue samples.