Microsatellite markers for the giant kelp Macrocystis pyrifera
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We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.
KeywordsMacrocystis pyrifera Giant kelp Microsatellites Macroalga Genetic diversity
We thank E. Hoaglund and T. Crombie for technical assistance. Financial support for this work was provided by the US National Science Foundation grant numbers OCE96-14091, OCE99-82105, and OCE06-20276 and by Fundação para a Ciência e Tecnologia pos-doctoral grant [SFRH/BPD/14945/2004 to F.A.], and grant MEGIKELP [PTDC/MAR/65461/2006].
- Alberto F (2009) MsatAllele_1.0: an R package to visualize the binning of microsatellite alleles. J Hered. doi: 10.1093/jhered/esn110
- Billote N, Lagoda PJL, Risterucci A et al (1999) Microsatellite enriched libraries: applied methodology for the development of ISSR markers in tropical crops. Fruits 54:277–288Google Scholar
- Graham MH, Vásquez JA, Buschmann AH (2007) Global ecology of the giant kelp Macrocystis: from ecotypes to ecossystems. Oceanogr Mar Biol Annu Rev 45:39–88Google Scholar
- Raymond M, Rousset F (1995) Genepop (version-1.2)—population-genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar