Conservation Genetics

, 10:1915 | Cite as

Microsatellite markers for the giant kelp Macrocystis pyrifera

  • Filipe Alberto
  • Allison Whitmer
  • Nélson C. Coelho
  • Mackenzie Zippay
  • Elena Varela-Alvarez
  • Peter T. Raimondi
  • Daniel C. Reed
  • Ester A. Serrão
Technical Note

Abstract

We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.

Keywords

Macrocystis pyrifera Giant kelp Microsatellites Macroalga Genetic diversity 

References

  1. Alberto F (2009) MsatAllele_1.0: an R package to visualize the binning of microsatellite alleles. J Hered. doi:10.1093/jhered/esn110
  2. Billote N, Lagoda PJL, Risterucci A et al (1999) Microsatellite enriched libraries: applied methodology for the development of ISSR markers in tropical crops. Fruits 54:277–288Google Scholar
  3. Coyer JA, Smith GJ, Andersen RA (2001) Evolution of Macrocystis spp. (Phaeophyceae) as determined by ITS1 and ITS2 sequences. J Phycol 37:574–585. doi:10.1046/j.1529-8817.2001.037001574.x CrossRefGoogle Scholar
  4. Graham MH (2004) Effects of local deforestation on the diversity and structure of Southern California giant kelp forest food webs. Ecosystems (NY, Print) 7:341–357. doi:10.1007/s10021-003-0245-6 Google Scholar
  5. Graham MH, Vásquez JA, Buschmann AH (2007) Global ecology of the giant kelp Macrocystis: from ecotypes to ecossystems. Oceanogr Mar Biol Annu Rev 45:39–88Google Scholar
  6. Koblizkova A, Dolezel J, Macas J (1998) Subtraction with 3′ modified oligonucleotides eliminates amplification artifacts in DNA libraries enriched for microsatellites. Biotechniques 25:32PubMedGoogle Scholar
  7. Raymond M, Rousset F (1995) Genepop (version-1.2)—population-genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  8. Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  9. Van Oosterhout C, Hutchinson WF, Wills DPM et al (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x CrossRefGoogle Scholar
  10. Varela-Alvarez E, Andreakis N, Lago-Leston A et al (2006) Genomic DNA isolation from green and brown algae (Caulerpales and Fucales) for microsatellite library construction. J Phycol 42:741–745. doi:10.1111/j.1529-8817.2006.00218.x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Filipe Alberto
    • 1
  • Allison Whitmer
    • 2
  • Nélson C. Coelho
    • 1
  • Mackenzie Zippay
    • 2
  • Elena Varela-Alvarez
    • 1
  • Peter T. Raimondi
    • 3
  • Daniel C. Reed
    • 2
  • Ester A. Serrão
    • 1
  1. 1.CCMAR, CIMAR-Laboratório AssociadoUniversity of AlgarveFaroPortugal
  2. 2.Marine Science InstituteUniversity of CaliforniaSanta BarbaraUSA
  3. 3.Department of Ecology and Evolutionary Biology, Center for Ocean Health, Long Marine LabUniversity of CaliforniaSanta CruzUSA

Personalised recommendations