Characterization of 26 microsatellite marker loci in the fossa (Cryptoprocta ferox)
The fossa (Cryptoprocta ferox) is one of eight carnivore species endemic to Madagascar. This species lives in the forests of Madagascar and is classified as vulnerable by the IUCN due to the loss and fragmentation of its natural habitat and the hunting pressure of local people. Twenty-six nuclear microsatellite loci were isolated from genomic DNA derived from a free-ranging fossa from Betampona, Madagascar. Population genetic parameters were estimated on 28 individuals from nine European zoos establishing a baseline to study the genetic health and diversity of captive fossas to support the European Breeding and Conservation Program (EEP). The marker suite averaged 4.4 alleles per locus with an average polymorphic information content of 0.478.
KeywordsGenetic markers Fossa Cryptoprocta ferox Eupleridae Madagascar Microsatellites
The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. This research was also supported by grants from the Ahmanson Foundation, which have provided the laboratory with three ABI automated DNA sequencers. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo/Madagascar Biodiversity and Biogeography Project. We thank the German Academic Exchange Service (DAAD) and the Fossa Fund (Zoo Duisburg) which supported the research stays of the author BRV. This project would not have been possible without the support of the zoos of Amsterdam (NL), Berlin (D), Bratislava (SL), Chemnitz (D), Colchester (UK), Duisburg (D), Frankfurt (D), Olomouc (CZ), Omaha (NE, USA) and Ustí nad Labem (CZ).
- Albignac R (1972) The carnivora of Madagascar. In: Battistini R, Richard-Vinard G (eds) Biogeography and ecology in Madagascar. W. Junk, The Hague, pp 667–682Google Scholar
- Bennett E (1833) Notice of a mammiferous animal from Madagascar, constituting a new form among the viverridous carnivora. Trans Zool Soc Lond 1:137–140Google Scholar
- Goudet J (1995) FSTAT, a computer program to test F-statistics. J Hered 86:85–486Google Scholar
- Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Updated from Goudet (1995)Google Scholar
- Hillis DM, Mable BK, Larson A, Davis SK, Zimmer EA (1996) Nucleic acids IV: sequencing and cloning. In: Hillis DM, Moritz C, Zimmer EA (eds) Molecular systematics, 2nd edn. Sinauer Associates Inc., Sunderland, pp 321–381Google Scholar
- IUCN (2008) 2008 IUCN Red List of Threatened Species. www.iucnredlist.org. Downloaded on 08 October 2008
- Moraga-Amador D, Farmerie BA, Brazeau D, Clark G (2001) “Tools for developing molecular markers. In: Interdisciplinary center for biotechnology research laboratory manual. The University of Florida Gainesville, Florida, pp 11–65Google Scholar
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms. J Hered 86:248–249Google Scholar
- Sambrook J, Fritch EF, Maniatus T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Press, New YorkGoogle Scholar
- Wemmer C, Wozencraft WC (2001) Civets, genets and mongooses. In: Macdonald D (ed) The new encyclopedia of mammals. Oxford University Press, Oxford, pp 122–131Google Scholar