Conservation Genetics

, 10:1195

Fifteen polymorphic microsatellite loci from Jamaican streamertail hummingbirds (Trochilus)

  • Stacey L. Lance
  • Cris Hagen
  • Travis C. Glenn
  • Robb T. Brumfield
  • Katherine Faust Stryjewski
  • Gary R. Graves
Technical Note

Abstract

We isolated and characterized 15 microsatellite loci from the endemic Jamaican streamertail hummingbird Trochilus polytmus. Loci were screened in 12 individuals of both T. polytmus and its sister species T. scitulus, also a Jamaican endemic. The number of alleles per locus ranged from 2 to 10, observed heterozygosity ranged from 0 to 1, and the probability of identity values ranged from 0.038 to 0.663. These new loci provide tools for characterizing the narrow hybrid zone between the two species.

Keywords

Black-billed streamertail Hummingbird Jamaica Microsatellite PCR primers SSR STR Red-billed streamertail Trochilus polytmus Trochilus scitulus 

References

  1. De Woody AJ, Schupp J, Kenefic L et al (2004) Universal method for producing ROX-labeled size standards suitable for automated genotyping. Biotechniques 37:348–350Google Scholar
  2. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) ‘Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008. doi:10.1093/nar/19.14.4008 PubMedCrossRefGoogle Scholar
  3. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol 8:92–94CrossRefGoogle Scholar
  4. Gill FB, Stokes FJ, Stokes C (1973) Contact zones and hybridization in the Jamaican Hummingbird Trochilus polytmus( L.). Condor 75:170–176. doi:10.2307/1365864 CrossRefGoogle Scholar
  5. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222. doi:10.1016/S0076-6879(05)95013-1 PubMedCrossRefGoogle Scholar
  6. Gosse PH (1847) The birds of Jamaica. Bentley, Wilson and Fley, LondonGoogle Scholar
  7. Graves GR (2009) Ontogeny of bill color in streamertail hummingbirds. J Caribb Ornithol 22Google Scholar
  8. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295. doi:10.1111/j.1471-8286.2005.01155.x CrossRefGoogle Scholar
  9. Rousset F (2008) genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106. doi:10.1111/j.1471-8286.2007.01931.x CrossRefGoogle Scholar
  10. Schuchmann K-L (1978) Allopatrische Artbildung bei der Kolibrigattung Trochilus. Ardea 66:156–172Google Scholar
  11. Schuchmann K-L (1999) Family Trochilidae. In: del Hoyo J, Elliott A, Sargatal J (eds) Handbook of the birds of the world, Barn-owls to hummingbirds, vol 5. Lynx Edicions, Barcelona, pp 468–680Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Stacey L. Lance
    • 1
    • 2
  • Cris Hagen
    • 2
  • Travis C. Glenn
    • 1
  • Robb T. Brumfield
    • 3
    • 4
  • Katherine Faust Stryjewski
    • 3
    • 4
    • 5
  • Gary R. Graves
    • 6
  1. 1.Department of Environmental Health ScienceUniversity of GeorgiaAthensUSA
  2. 2.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  3. 3.Museum of Natural ScienceLouisiana State UniversityBaton RougeUSA
  4. 4.Department of Biological SciencesLouisiana State UniversityBaton RougeUSA
  5. 5.Department of BiologyBoston UniversityBostonUSA
  6. 6.Department of Vertebrate Zoology MRC-116, National Museum of Natural HistorySmithsonian InstitutionWashingtonUSA

Personalised recommendations