Fifteen polymorphic microsatellite loci from Jamaican streamertail hummingbirds (Trochilus)
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We isolated and characterized 15 microsatellite loci from the endemic Jamaican streamertail hummingbird Trochilus polytmus. Loci were screened in 12 individuals of both T. polytmus and its sister species T. scitulus, also a Jamaican endemic. The number of alleles per locus ranged from 2 to 10, observed heterozygosity ranged from 0 to 1, and the probability of identity values ranged from 0.038 to 0.663. These new loci provide tools for characterizing the narrow hybrid zone between the two species.
KeywordsBlack-billed streamertail Hummingbird Jamaica Microsatellite PCR primers SSR STR Red-billed streamertail Trochilus polytmus Trochilus scitulus
Research and export permits were issued by the National Environmental Planning Agency, Kingston (Yvette Strong, Andrea Donaldson, Ricardo Miller) and the Jamaica Conservation and Development Trust (Susan Otuokan). GRG thanks Brian Schmidt and Errol Francis for field assistance and Catherine Levy (Kingston) and Susan Koenig and Michael Schwartz (Windsor Research Centre) for logistical assistance. This work was supported by Department of Energy award DE-FC09-07SR22506, James Bond and Alexander Wetmore funds from the Smithsonian Institution to GRG, and NSF grant DEB-0543562 to RTB.
This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.
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