Quick DNA preparation for direct PCR in marine invertebrates and fishes
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A modified protocol was developed for extracting DNA and direct PCR from tissues of marine invertebrates and finfishes. Protocol represented combination of classical lysis using a modified buffer system comprising KCl, Tris buffer and MgCl2. The DNA obtained was quantified and tested by mtDNA-PCR and RAPD. Test results indicated usefulness of this method in studies involving screening of large numbers of samples, such as evolutionary, forensic and population studies.
KeywordsMarine invertebrates Fishes DNA extraction Direct-PCR Population genetics Molecular taxonomy
Reynold Peter acknowledges financial support from the Department of Biotechnology, Government of India. We acknowledge the help rendered by Mr. Jobin Jose for his help in improving the manuscript.
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