A modified protocol was developed for extracting DNA and direct PCR from tissues of marine invertebrates and finfishes. Protocol represented combination of classical lysis using a modified buffer system comprising KCl, Tris buffer and MgCl2. The DNA obtained was quantified and tested by mtDNA-PCR and RAPD. Test results indicated usefulness of this method in studies involving screening of large numbers of samples, such as evolutionary, forensic and population studies.
Marine invertebrates Fishes DNA extraction Direct-PCR Population genetics Molecular taxonomy
This is a preview of subscription content, log in to check access.
Reynold Peter acknowledges financial support from the Department of Biotechnology, Government of India. We acknowledge the help rendered by Mr. Jobin Jose for his help in improving the manuscript.
Fode-Vaughan KA, Wimpee CF, Remsen CC et al (2001) Detection of bacteria in environmental samples by direct PCR without DNA extraction. Biotechniques 31:598–607PubMedGoogle Scholar
Kotlowski R, Martin A, Ablordey AK et al (2004) One- tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish. J Med Microbiol 53:927–933. doi:10.1099/jmm.0.45593-0PubMedCrossRefGoogle Scholar
Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. CSH Laboratory Press, Cold Spring HarborGoogle Scholar