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Conservation Genetics

, 10:1159 | Cite as

Quick DNA preparation for direct PCR in marine invertebrates and fishes

  • Reynold Peter
  • Siby Philip
  • Allen Benziger
Technical Note

Abstract

A modified protocol was developed for extracting DNA and direct PCR from tissues of marine invertebrates and finfishes. Protocol represented combination of classical lysis using a modified buffer system comprising KCl, Tris buffer and MgCl2. The DNA obtained was quantified and tested by mtDNA-PCR and RAPD. Test results indicated usefulness of this method in studies involving screening of large numbers of samples, such as evolutionary, forensic and population studies.

Keywords

Marine invertebrates Fishes DNA extraction Direct-PCR Population genetics Molecular taxonomy 

Notes

Acknowledgments

Reynold Peter acknowledges financial support from the Department of Biotechnology, Government of India. We acknowledge the help rendered by Mr. Jobin Jose for his help in improving the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Marine Biotechnology DivisionCentral Marine Fisheries Research InstituteErnakulamIndia
  2. 2.Department of ZoologyUniversity of KeralaThiruvananthapuramIndia
  3. 3.Zebra Fish Genomics LabInstitute of Genomics and Integrative BiologyNew DelhiIndia

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