Development and characterization of new microsatellite loci from Chinese mitten crab (Eriocheir sinensis)
- 106 Downloads
A set of new microsatellite loci were isolated from Chinese mitten crab and characterized in two Chinese mitten crab populations. The number of the alleles ranged from 3 to 8 and observed and expected heterozygosity varied from 0.0870 to 0.9565 and 0.3092 to 0.8367 in each population, respectively. Loci HLJEsin1, HLJEsin4, HLJEsin30 in CLN population and HLJEsin6 in CJS population presented significant deviation from Hardy–Weinberg equilibrium (PHWE < 0.001). These markers will be useful for population studies.
KeywordsMicrosatellites Germplasm resource Chinese mitten crab Primers
This work was funded by Key Project of Chinese National Programs for Foundational Research and Development (2004CB117405).
- Raymond M, Rousset F (1995) GENEPOP (vers. 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
- Yeh FC, Yang R, Boyle TJ, Xiyan JM (2000) PopGene32. Microsoft window-based freeware for population genetic analysis, version 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, EdmontonGoogle Scholar