Characterization of 8 polymorphic microsatellite loci in the neotropical ant-garden ant, Camponotus femoratus (Fabricius)
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Camponotus femoratus is an abundant and behaviorally dominant ant in lowland Amazonian rainforests, where this species participates in a complex and obligate seed-dispersal mutualism. C. femoratus typically cohabits with another ant species Crematogaster levior in an apparently amiable but poorly understood interaction. Despite these outstanding characteristics, the population genetics and dispersal patterns of C. femoratus are unknown. We isolated eight polymorphic microsatellite loci for C. femoratus from a genomic library enriched for di-, tri-, and tetra-nucleotide repeats. We detected 2 to 17 alleles per locus, with levels of observed heterozygosity ranging from 0.286 to 0.714.
KeywordsCamponotus Formicidae Di-nucleotide microsatellite Tri-nucleotide microsatellite Tetra-nucleotide microsatellite
We thank John Longino for ant identification and Jeniffer Katherine Alvarez Baca for field assistance. This study was partially supported by a National Science Foundation predoctoral fellowship (EY), a U.S. Department of Education GAANN fellowship (EY), an Amazon Conservation Association graduate research grant (EY), a Sigma Xi Grant in Aid of Research (EY), a North Carolina Entomological Society travel grant (EY), the North Carolina State University Office of International Affairs (CS), grant number 2004-35302-14880 from the National Research Initiative of the USDA Cooperative State Research, and the Blanton J. Whitmire endowment at North Carolina State University. Permission to work and collect in the Los Amigos conservation concession was granted by the Intstituto Nacional de Recursos Naturales (INRENA) of Perú.
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