Eight novel microsatellite DNA markers in Rhodiola rosea L.
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An enrichment procedure was used to develop eight di- and tri-microsatellites for Rhodiola rosea L., whose root extract is used as a powerful adaptogen. In order to evaluate the polymorphism and allelic variation at each locus, 48 individuals sampled in two different locations in Trentino region (Italy) were analyzed. All loci were polymorphic, showing 2–5 alleles per locus and an observed heterozygosity ranging from 0.09 to 0.76. HWE test shows significant deviation for five loci in two different populations. No linkage disequilibrium among loci was observed. These SSR markers are being used for the study of the genetic variation within and among populations in the Trentino Alps.
KeywordsGolden root SSR markers Adaptogen Medicinal plant Population genetics
This research was supported by the project PARMA (Piante Alimentari, aRomatiche e Medicinali Alpine) financed by the Autonomous Province of Trento. The authors would like to thank Nicola Aiello for his help in the collection of the different populations.
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