Characterization and multiplex genotyping of 16 polymorphic microsatellite loci in the endangered bumble-bee bat, Craseonycteris thonglongyai (Chiroptera: Craseonycteridae)
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The bumble-bee bat (Craseonycteris thonglongyai) is an endangered species with a limited distribution range and a suspected declining population. Of the 30 microsatellites developed and tested in C. thonglongyai, 16 were amplified in two multiplex polymerase chain reactions (PCRs). Based on genotypes of 64 individuals from two populations, expected heterozygosities, averaged 0.50 in Myanmar and 0.61 in Thailand. Only one pair of loci showed linkage disequilibrium in one population and no deviation from Hardy–Weinberg equilibrium was detected. This set of 16 microsatellites will be useful to study population structure and gene flow in C. thonglongyai.
KeywordsCraseonycteris thonglongyai Chiroptera Microsatellites Endangered species Population structure
Thanks to everyone who participated in the fieldwork and to the monks who allowed us to access the caves. Thanks to the IRD UR 178 and Mahidol University for their ongoing collaboration and support on this project. Thanks to Eric Petit for his comments on the manuscript. In Ireland, we would like to thank Science Foundation Ireland (grant RFP-GEN0056) and the Royal Irish Academy/Royal Society for funding this project.
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