Characterization and multiplex genotyping of 16 polymorphic microsatellite loci in the endangered bumble-bee bat, Craseonycteris thonglongyai (Chiroptera: Craseonycteridae)
- 174 Downloads
The bumble-bee bat (Craseonycteris thonglongyai) is an endangered species with a limited distribution range and a suspected declining population. Of the 30 microsatellites developed and tested in C. thonglongyai, 16 were amplified in two multiplex polymerase chain reactions (PCRs). Based on genotypes of 64 individuals from two populations, expected heterozygosities, averaged 0.50 in Myanmar and 0.61 in Thailand. Only one pair of loci showed linkage disequilibrium in one population and no deviation from Hardy–Weinberg equilibrium was detected. This set of 16 microsatellites will be useful to study population structure and gene flow in C. thonglongyai.
KeywordsCraseonycteris thonglongyai Chiroptera Microsatellites Endangered species Population structure
Thanks to everyone who participated in the fieldwork and to the monks who allowed us to access the caves. Thanks to the IRD UR 178 and Mahidol University for their ongoing collaboration and support on this project. Thanks to Eric Petit for his comments on the manuscript. In Ireland, we would like to thank Science Foundation Ireland (grant RFP-GEN0056) and the Royal Irish Academy/Royal Society for funding this project.
- Bates PJJ, Nwe T, Swe KM, Hla Bu SS (2001) Further new records of bats from Myanmar (Burma) including Craseonycteris thonglongyai Hill 1974 (Chiroptera: Craseonycteridae). Acta Chiropt 3:33–41Google Scholar
- Hill JE (1974) A new family, genus and species of bat (Mammalia: Chiroptera) from Thailand. Bull Br Mus Nat Hist Zool 27:301–336Google Scholar
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
- Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
- Yokubol M (2000) Habitat use and the population trend of kitti’s hog-nosed bats (Craseonycteris thonglongyai) in disturbed habitats in Western Thailand. Master’s Thesis (Environmental Biology), Faculty of Graduate Studies, Mahidol University, ThailandGoogle Scholar