Conservation Genetics

, Volume 10, Issue 4, pp 1073–1076 | Cite as

Characterization and multiplex genotyping of 16 polymorphic microsatellite loci in the endangered bumble-bee bat, Craseonycteris thonglongyai (Chiroptera: Craseonycteridae)

  • Sébastien J. Puechmaille
  • Piyathip Piyapan
  • Medhi Yokubol
  • Meriadeg Ar Gouilh
  • Khin Mie Mie
  • Paul J. Bates
  • Chutamas Satasook
  • Tin Nwe
  • Si Si Hla Bu
  • Iain Mackie
  • Emma C. Teeling
Technical Note

Abstract

The bumble-bee bat (Craseonycteris thonglongyai) is an endangered species with a limited distribution range and a suspected declining population. Of the 30 microsatellites developed and tested in C. thonglongyai, 16 were amplified in two multiplex polymerase chain reactions (PCRs). Based on genotypes of 64 individuals from two populations, expected heterozygosities, averaged 0.50 in Myanmar and 0.61 in Thailand. Only one pair of loci showed linkage disequilibrium in one population and no deviation from Hardy–Weinberg equilibrium was detected. This set of 16 microsatellites will be useful to study population structure and gene flow in C. thonglongyai.

Keywords

Craseonycteris thonglongyai Chiroptera Microsatellites Endangered species Population structure 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Sébastien J. Puechmaille
    • 1
  • Piyathip Piyapan
    • 2
  • Medhi Yokubol
    • 2
  • Meriadeg Ar Gouilh
    • 3
    • 4
  • Khin Mie Mie
    • 5
  • Paul J. Bates
    • 6
  • Chutamas Satasook
    • 2
  • Tin Nwe
    • 5
  • Si Si Hla Bu
    • 7
  • Iain Mackie
    • 8
  • Emma C. Teeling
    • 1
  1. 1.School of Biological and Environmental SciencesUniversity College DublinDublin 4Ireland
  2. 2.Department of BiologyPrince of Songkla UniversityHat YaiThailand
  3. 3.Muséum National d’Histoire NaturelleParisFrance
  4. 4.Faculty of ScienceMahidol UniversityBangkokThailand
  5. 5.Zoology DepartmentYangon UniversityYangonMyanmar
  6. 6.Harrison InstituteSevenoaksUK
  7. 7.Department of ZoologyMandalay University (formerly of Yangon University)MandalayMyanmar
  8. 8.Department of BiologyUniversity of Aberdeen, IBESAberdeenUK

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