Conservation Genetics

, Volume 10, Issue 4, pp 1013–1015 | Cite as

Eight SSR loci from Oyster Crassostrea gigas EST database and cross-species amplification in C. plicatula

  • Xuemei Qiu
  • Shaozhen Liu
  • Xiuli Wang
  • Xiangying Meng
Technical Note

Abstract

Oyster (Crassostrea plicatula) is widely distributed in coastal areas of China. We developed and evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Crassostrea gigas and to amplify EST-SSR in C. plicatula. Characteristics of eight EST-SSR loci were investigated using 37 wild-type C. plicatula individuals. The number of alleles per locus ranged from four to six. The observed heterozygosity (H O) ranged from 0.1892 to 0.7027 (0.3919 on average) and the expected heterozygosity (H E) ranged from 0.6068 to 0.7656 (0.7039 on average) (P < 0.05). The average observed heterozygosity was much lower than the average expected heterozygosity. All loci except C15 departed from Hardy-Weinberg equilibrium (P < 0.05) significantly. Contribution of the eight EST-SSR primers presented here will provide necessary and powerful molecular tools for management and conservation studies on the species of oysters in the future.

Keywords

EST SSRs Oyster Crassostrea plicatula Crassostrea gigas 

Notes

Acknowledgements

This project was supported by the Scientific Foundation of the Educational Department of Liaoning Province (Grant No. 20060187)

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Xuemei Qiu
    • 1
  • Shaozhen Liu
    • 1
  • Xiuli Wang
    • 1
  • Xiangying Meng
    • 1
  1. 1.College of Life Sciences and BiotechnologyDalian Fisheries UniversityShahekou District, DalianChina

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