Isolation and charaterization of 12 dinucleotide microsatellite loci from Belenger’s jewfish (Johnius belengerii Cuvier 1830)
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Abstract
We describe the first isolation of 12 polymorphic microsatellite markers from Belenger’s jewfish (Johnius belengnerii Cuvier 1830). From a (GT)n-enriched genomic library, 54 microsatellites were selected for designing microsatellite primers, of which 36 gave working primer pairs. 12 of these loci were polymorphic in a test population of 21 individuals with alleles ranging from 3 to 18, and expected and observed heterozygosities from 0.5772 to 0.9449 and from 0.4286 to 0.9231, respectively. No significant linkage disequilibrium between pairs of loci was found, however, loci Jobe24 significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate population structure in Belenger’s jewfish.
Keywords
Microsatellite Belenger’s jewfish Johnius belengnerii Cuvier Enriched genomic libraryNotes
Acknowledgment
This study was supported by State 863 High-technology R&D project of China (2006AA10A403), Taishan Scholar Project of Shandong Province, National Public Platform of Science & Technology Information Resources (2005DKA30470-06) and scientific foundation of YSFRI, CAFS (2007-qn-13).
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