Conservation Genetics

, Volume 10, Issue 3, pp 785–787

Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotonacurzoniae)

Technical Note


High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium. This information provides efficient tools to allow future parentage studies.


Magnetic beads enrichment Microsatellite Parentage analysis Plateau pika Polymorphism 


  1. Evanno GRS, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620. doi:10.1111/j.1365-294X.2005.02553.x PubMedCrossRefGoogle Scholar
  2. Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86:485Google Scholar
  3. Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27(3):500–502, 504–507Google Scholar
  4. Jin L, Zhong Y, Chakraborty R (1994) The exact numbers of possible microsatellite motifs. Am J Hum Genet 55:582PubMedGoogle Scholar
  5. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959PubMedGoogle Scholar
  6. Raymond MRF (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  7. Raymond M, Rousset F (2003) Genepop, version 3. 4. Population genetics software for exact tests and ecumenicism. Updated from Raymond and Rousset (1995)Google Scholar
  8. Smith AT, Foggin JM (1999) The plateau pika (Ochotona curzoniae) is a keystone species for biodiversity on the Tibetan plateau. Anim Conserv 2:235–240. doi:10.1111/j.1469-1795.1999.tb00069.x CrossRefGoogle Scholar
  9. Van Oosterhout C, Hutchinson WF, Wills DP, Shipley P (2004) PROGRAM NOTE: micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Graduate School of the Chinese Academy of SciencesBeijingChina
  2. 2.Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of BiologyThe Chinese Academy of SciencesXining, QinghaiChina
  3. 3.Key Laboratory of Genome Information and Sciences, Beijing Institute of GenomicsThe Chinese Academy of SciencesBeijingChina

Personalised recommendations