Conservation Genetics

, Volume 10, Issue 3, pp 785–787

Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotonacurzoniae)

Technical Note

Abstract

High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium. This information provides efficient tools to allow future parentage studies.

Keywords

Magnetic beads enrichment Microsatellite Parentage analysis Plateau pika Polymorphism 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Graduate School of the Chinese Academy of SciencesBeijingChina
  2. 2.Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of BiologyThe Chinese Academy of SciencesXining, QinghaiChina
  3. 3.Key Laboratory of Genome Information and Sciences, Beijing Institute of GenomicsThe Chinese Academy of SciencesBeijingChina

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