Conservation Genetics

, Volume 10, Issue 4, pp 963–980

253 Novel polymorphic microsatellites for the saltwater crocodile (Crocodylus porosus)

  • Lee G. Miles
  • Sally R. Isberg
  • Chris Moran
  • Cris Hagen
  • Travis C. Glenn
Technical Note

DOI: 10.1007/s10592-008-9600-7

Cite this article as:
Miles, L.G., Isberg, S.R., Moran, C. et al. Conserv Genet (2009) 10: 963. doi:10.1007/s10592-008-9600-7

Abstract

Genomic elucidation and mapping of novel organisms requires the generation of large genetic resources. In this study, 253 novel and polymorphic microsatellite loci were isolated and characterized for the saltwater crocodile (Crocodylus porosus) by constructing libraries enriched for microsatellite DNA. All markers were evaluated on animals obtained from Darwin Crocodile Farm in the Northern Territory, Australia, and are intended for future use in the construction of a genetic-linkage map for the saltwater crocodile. The 253 loci yielded an average of 4.12 alleles per locus, and those selected for mapping had an average polymorphic information content (PIC) of 0.425.

Keywords

Estuarine crocodile Reptile SSRs Primers Tetra-nucleotide repeats Tri-nucleotide repeats Di-nucleotide repeats Enrichment 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Lee G. Miles
    • 1
  • Sally R. Isberg
    • 1
    • 2
  • Chris Moran
    • 1
  • Cris Hagen
    • 3
  • Travis C. Glenn
    • 3
    • 4
  1. 1.Faculty of Veterinary ScienceUniversity of SydneySydneyAustralia
  2. 2.Porosus Pty LtdPalmerstonAustralia
  3. 3.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  4. 4.Department of Environmental Health ScienceUniversity of GeorgiaAthensUSA

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