Conservation Genetics

, Volume 10, Issue 3, pp 577–580

Tetranucleotide markers from the loggerhead sea turtle (Caretta caretta) and their cross-amplification in other marine turtle species

  • Brian M. Shamblin
  • Brant C. Faircloth
  • Mark G. Dodd
  • Dean A. Bagley
  • Llewellyn M. Ehrhart
  • Peter H. Dutton
  • Amy Frey
  • Campbell J. Nairn
Technical Note

DOI: 10.1007/s10592-008-9573-6

Cite this article as:
Shamblin, B.M., Faircloth, B.C., Dodd, M.G. et al. Conserv Genet (2009) 10: 577. doi:10.1007/s10592-008-9573-6

Abstract

The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea turtle. We tested primers using samples from 22 females that nested at Melbourne Beach, Florida (USA). Primer pairs yielded an average of 11.2 alleles per locus (range of 4–24), an average observed heterozygosity of 0.83 (range 0.59–0.96), and an average polymorphic information content of 0.80 (range 0.62–0.94). We also demonstrate the utility of these primers, in addition to primers for 15 loci previously described, for amplifying microsatellite loci in four additional species representing the two extant marine turtle families: olive ridley (Lepidochelys olivacea), hawksbill (Eretmochelys imbricata), green turtle (Chelonia mydas), and leatherback (Dermochelys coriacea).

Keywords

Caretta caretta Loggerhead turtle Microsatellites Cross-amplification 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Brian M. Shamblin
    • 1
  • Brant C. Faircloth
    • 1
  • Mark G. Dodd
    • 2
  • Dean A. Bagley
    • 3
  • Llewellyn M. Ehrhart
    • 3
    • 4
  • Peter H. Dutton
    • 5
  • Amy Frey
    • 5
  • Campbell J. Nairn
    • 1
  1. 1.D. B. Warnell School of Forestry and Natural ResourcesThe University of GeorgiaAthensUSA
  2. 2.Wildlife Resources DivisionGeorgia Department of Natural ResourcesBrunswickUSA
  3. 3.Department of BiologyThe University of Central FloridaOrlandoUSA
  4. 4.Hubbs-SeaWorld Research InstituteOrlandoUSA
  5. 5.NOAA-FisheriesSouthwest Fisheries Science CenterLa JollaUSA

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