Ten microsatellite loci from Northern Bobwhite (Colinus virginianus)
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Ecological studies using microsatellite data often require the selection of an optimal marker set for use in parentage and relatedness inference. Commonly, this requires a candidate pool of microsatellite markers from which several are selected to ensure data are acquired efficiently and accurately. We developed 10 microsatellite loci for use with Northern Bobwhite (Colinus virginianus) and tested these loci using individuals collected from two distinct populations in GA and VA. Our new markers yielded seven alleles/locus (range: 2–16) in the Georgia population and six alleles/locus (range: 2–13) in the Virginia population. Exclusionary power of all markers in each population with both parents unknown was >0.98. These microsatellite loci should be combined with previously developed markers to select an optimal set for use in subsequent analyses of parentage and relatedness.
KeywordsMicrosatellites SSRs Galliformes Northern Bobwhite Colinus virginianus
Financial support was provided by: Tall Timbers Research Station and Land Conservancy’s Quail Research Initiative, Daniel B. Warnell School of Forestry and Natural Resources, and McIntire-Stennis projects GEO-0100-MS and GEO-0136-MS. Capture and handling of bobwhites in Georgia were approved by the Georgia Department of Natural Resources (permits: 29-WMB-00–105 and 29-WMB-03–38), and the University of Georgia Institutional Animal Care and Use Committee (approvals: A990028M1, A990028C1, and A200310109-0). Bobwhite wings collected in Virginia were salvaged from hunter-killed animals as part of a Virginia Department of Game and Inland Fisheries program. The authors thank Patrick Cook and Steve Capel for the donation of these wings.
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