Conservation Genetics

, Volume 10, Issue 1, pp 169–176 | Cite as

High genetic diversity among fossorial lizard populations (Anniella pulchra) in a rapidly developing landscape (Central California)

Research Article

Abstract

California is a biodiversity hotspot facing unbridled human population growth, especially in Central California. One of the poorly known, sensitive species in this area is the California legless lizard (Anniella pulchra), a fossorial worm-like reptile. We report mt and nuDNA sequences from 69 museum-vouchered samples of Anniella (A. pulchra and its sister species A. geronimensis) from 48 localities. Our genetic survey reveals substantially more genetic diversity within A. pulchra than previously reported. Our two independently evolving markers (mt and nuDNA) reveal five major lineages of A. pulchra. Two of the five major lineages of A. pulchra correspond to a north-south split found in other widespread California reptiles. These northern and southern clades also correspond to a previous study showing variation in chromosomal number. Unlike most other Californian reptiles, A. pulchra has major genetic lineages that are endemic to Central California including two that are endemic to the San Joaquin Valley and Carrizo Plain. Although A. pulchra is threatened throughout its range, the distinct San Joaquin lineages are seriously imperiled by urban sprawl. Some of the localities for the newly recognized genetic lineages have already been destroyed by development.

Keywords

Anguidae Phylogeny Conservation Genetics San Joaquin Valley 

References

  1. Ast JC (2001) Mitochondrial DNA evidence and evolution in Varanoidea (Squamata). Cladistics 17:211–226CrossRefGoogle Scholar
  2. Bezy RL, Gorman GC, Kim YK, Wright JW (1977) Chromosomal and genetic divergence in the fossorial lizards of the family Anniellidae. Syst Zool 26:57–71CrossRefGoogle Scholar
  3. Bezy RL, Wright JW (1971) Karyotopic variation and relationships of the California legless lizard, Anniella pulchra Gray (Reptilia: Anniellidae). Herpetol Rev 3:71–72Google Scholar
  4. Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1659PubMedCrossRefGoogle Scholar
  5. Feldman CR, Spicer GS (2006) Comparative phylogeography of woodland reptiles in California: repeated patterns of cladogenesis and population expansion. Mol Ecol 15:2201–2222PubMedCrossRefGoogle Scholar
  6. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  7. Fischer JG (1885) Uber eine zweite Art der Gattung Anniella Gray. Abhandl Naturwiss Verein Hamburg 9:9–10Google Scholar
  8. Gray JE (1852) Descriptions of several new genera of reptiles, principally from the collection of the H.M.S. Herald. Ann Mag Nat Hist Ser 2 10:437–440Google Scholar
  9. Great Valley Center (2007) Our valley. Our choice. Great Valley Center, ModestoGoogle Scholar
  10. Hall CA (2002) Nearshore marine paleoclimatic regions, increasing zoogeographic provinciality, mollsucan extinctions, and paleoshorelines, California: Late Oligocene (27 Ma) to Late Pliocene (2.5 Ma). Geol Soc Am Spec Pap 357Google Scholar
  11. Hunt LE (1983) A nomenclatural rearrangement of the genus Anniella (Sauria: Anniellidae). Copeia 1983:79–89CrossRefGoogle Scholar
  12. Hunt LE (1984) Morphological variation in the fossorial lizard Anniella. Master’s Thesis, University of KansasGoogle Scholar
  13. Jockusch EL, Wake DB (2002) Falling apart and merging: diversification of slender salamanders (Plethodontidae: Batrachoseps) in the American West. Biol J Linn Soc 76:361–391CrossRefGoogle Scholar
  14. Jockusch EL, Wake DB, Yanev KP (1998) New species of slender salamanders, Batrachoseps (Amphibia: Plethodontidae), from the Sierra Nevada of California. Nat Hist Mus Los Angeles Co Sci Ser 472:1–17Google Scholar
  15. Kolankiewicz L, Beck R (2000) Sprawl in California. NumbersUSA, ArlingtonGoogle Scholar
  16. Leache AD, Mulcahy DM (2007) Phylogeny, divergence times and species limits of spiny lizards (Sceloporus magister species group) in western North American deserts and Baja California. Molec Ecol 16:5216–5233PubMedCrossRefGoogle Scholar
  17. Macey JR, Schulte JA, Larson A, Tuniyev BS, Orlov N, Papenfuss TJ (1999) Molecular phylogenetics, tRNA evolution, and historical biogeography in anguid lizards and related taxonomic families. Mol Phylogenet Evol 12:250–272PubMedCrossRefGoogle Scholar
  18. Myers N, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858PubMedCrossRefGoogle Scholar
  19. Noonan BP, Chippendale PT (2006) Dispersal and vicariance: the complex evolutionary history of boid snakes. Mol Phylogenet Evol 40:347–358PubMedCrossRefGoogle Scholar
  20. Papenfuss TJ, Macey JR, Schulte JA (2001) A new lizard species in the genus Xantusia. Pap Nat Hist Mus Univ Kansas 23:1–9Google Scholar
  21. Parham JF, Irmis RB (2008) Caveats on the use of fossil calibrations for molecular dating. Am Nat 171:132–136PubMedCrossRefGoogle Scholar
  22. Parham JF, Macey JR, Papenfuss TJ, Feldman CR, Türkozan O, Polymeni R, Boore JL (2006) The phylogeny of Mediterranean tortoises and their close relatives based on complete mitochondrial genome sequences from museum specimens. Mol Phylogenet Evol 38:50–64PubMedCrossRefGoogle Scholar
  23. Pearse DE, Pogson GH (2000) Parallel evolution of the melanic form of the California legless lizard, Anniella pulchra, inferred from mitochondrial sequence variation. Evolution 54:1041–1046PubMedGoogle Scholar
  24. Posada D, Crandall KA (1998) Modletest: testing the model of DNA substitution. Bioinformatics 14:817–818PubMedCrossRefGoogle Scholar
  25. PPIC (2006) CA2025—California’s future population. Public Policy Institute of California, SacramentoGoogle Scholar
  26. Rissler LJ, Hijmans RJ, Graham CH, Moritz C, Wake DB (2006) Phylogeographic lineages and species comparisons in conservation analyses: A case study of California Herpetofauna. Am Nat 167:655–666PubMedCrossRefGoogle Scholar
  27. Shaw CE (1940) A new species of legless lizard from San Geronimo Island, Lower California, Mexico. Trans San Diego Soc Nat Hist 9:225–228Google Scholar
  28. Spinks PQ, Shaffer HB (2005) Range-wide molecular analysis of the western pond turtle (Emys marmorata): cryptic variation, isolation by distance, and their conservation implications. Mol Ecol 14:2047–2064PubMedCrossRefGoogle Scholar
  29. Stamatakis A (2006a) Phylogenetic models of rate heterogeneity: a high performance computing perspective. Parallel and Distributed Processing Symposium, 2006. doi:10.1109/IPDPS.2006.1639535
  30. Stamatakis A (2006b) RAxML-VI-HPC: maximum likelihood based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690PubMedCrossRefGoogle Scholar
  31. Swofford DL (2002) PAUP*: Phylogenetic analysis using parsimony *(and other methods), version 4.0b10. Sinauer Associates, SunderlandGoogle Scholar
  32. Wake DB (2006) Problems with species: patterns and processes of species formation in salamanders. Ann Mo Bot Gard 93:8–23CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of HerpetologyCalifornia Academy of SciencesSan FranciscoUSA
  2. 2.Museum of PaleontologyUniversity of CaliforniaBerkeleyUSA
  3. 3.Museum of Vertebrate ZoologyUniversity of CaliforniaBerkeleyUSA

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