Conservation Genetics

, Volume 9, Issue 6, pp 1673–1676 | Cite as

A set of polymorphic nuclear intron markers for conservation genetics and phylogeography of Euphorbia species (Pedilanthus clade)

  • R. C. Garrick
  • C. A. Meadows
  • A. N. Nicolas
  • J. D. Nason
  • R. J. Dyer
Technical Note

Abstract

We developed seven nuclear intron markers for Euphorbia lomelii. New exon-primed intron-crossing (EPIC) oligonucleotides were used for initial amplification and sequencing, then locus-specific primers and restriction-fragment-length polymorphism genotyping assays were designed. Loci showed no significant deviation from Hardy–Weinberg and linkage equilibrium, and they cross-amplify in at least three congeneric species.

Keywords

Co-dominant loci Euphorbiaceae Population structure Phylogeny Sonoran Desert 

Notes

Acknowledgements

We acknowledge National Science Foundation grant DEB-0543102 to RJD and JDN for funding, and. Ivalú Cacho for providing DNA for cross-species testing.

Supplementary material

10592_2008_9521_MOESM1_ESM.pdf (199 kb)
(PDF 199 kb)

References

  1. Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50Google Scholar
  2. Lewis CE, Doyle JJ (2001) Phylogenetic utility of the nuclear gene malate synthase in the palm family (Asteraceae). Mol Phylogenet Evol 19:409–420PubMedCrossRefGoogle Scholar
  3. Olson ME, Lomelí JA, Cacho NI (2005) Extinction threat in the Pedilanthus clade (Euphorbia, Euphorbiaceae), with special reference to the recently rediscovered E. conzattii (P. pulchellus). Am J Bot 92:634–641CrossRefGoogle Scholar
  4. Saad FA, Halliger B, Müller CR, Roberts RG, Danieli GA (1994) Single base substitutions are detected by double strand conformation analysis. Nucleic Acids Res 22:4352–4353PubMedCrossRefGoogle Scholar
  5. Sternburg C, Rodriguez E (1982) Hydrocarbons from Pedilanthus macrocarpus (Euphorbiaceae) of Baja California and Sonora, Mexico. Am J Bot 69:214–218CrossRefGoogle Scholar
  6. Vincze T, Posfai J, Roberts RJ (2003) NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res 31:3688–3691PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • R. C. Garrick
    • 1
  • C. A. Meadows
    • 1
  • A. N. Nicolas
    • 1
  • J. D. Nason
    • 2
  • R. J. Dyer
    • 1
  1. 1.Department of BiologyVirginia Commonwealth UniversityRichmondUSA
  2. 2.Department of Ecology, Evolution and Organismal BiologyIowa State UniversityAmesUSA

Personalised recommendations