A set of polymorphic nuclear intron markers for conservation genetics and phylogeography of Euphorbia species (Pedilanthus clade)
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We developed seven nuclear intron markers for Euphorbia lomelii. New exon-primed intron-crossing (EPIC) oligonucleotides were used for initial amplification and sequencing, then locus-specific primers and restriction-fragment-length polymorphism genotyping assays were designed. Loci showed no significant deviation from Hardy–Weinberg and linkage equilibrium, and they cross-amplify in at least three congeneric species.
KeywordsCo-dominant loci Euphorbiaceae Population structure Phylogeny Sonoran Desert
We acknowledge National Science Foundation grant DEB-0543102 to RJD and JDN for funding, and. Ivalú Cacho for providing DNA for cross-species testing.
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