Conservation Genetics

, Volume 9, Issue 6, pp 1665–1668 | Cite as

Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L.

  • Marta Dubreuil
  • Federico Sebastiani
  • Maria Mayol
  • Santiago C. González-Martínez
  • Miquel Riba
  • Giovanni G. Vendramin
Technical Note


Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals from 5 different populations (10–26 samples per population) were used to estimate genetic diversity parameters. High levels of genetic diversity, with values ranging from 0.533 to 0.929 (6–28 alleles per SSR) were found. No linkage disequilibrium between pairs of loci was detected. All loci but one showed significant departures from Hardy–Weinberg equilibrium. Excess of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical scales and to monitor gene flow.


Taxus baccata Microsatellite markers Genetic diversity Inbreeding 



This research was supported by funds from Spanish Ministry of Education and Science (Project CGL2004-00223/BOS) and the Generalitat de Catalunya (Emergent Research Group 2005SGR00381), and by a Ph.D. grant from DURSI (Departament d’Universitats, Recerca i Societat de la Informació; Generalitat de Catalunya) to M. Dubreuil (2004FI01255).


  1. Collins D, Mill RR, Moller M (2003) Species separation of Taxus baccata, T. canadensis and T. cuspidata (Taxaceae) and origins of their reputed hybrids inferred from RAPD and cpDNA data. Am J Bot 90:175–182CrossRefGoogle Scholar
  2. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21CrossRefGoogle Scholar
  3. Edwards KJ, Barker JHA, Daly A, Jones C, Karp A (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20:758–760PubMedGoogle Scholar
  4. Hilfiker K, Gugerli F, Schutz JP, Rotach P, Holderegger R (2004a) Low RAPD variation and female-biased sex ratio indicate genetic drift in small populations of the dioecious conifer Taxus baccata in Switzerland. Conserv Genet 5:357–365CrossRefGoogle Scholar
  5. Hilfiker K, Holderegger R, Rotach P, Gugerli F (2004b) Dynamics of genetic variation in Taxus baccata: local versus regional perspectives. Can J Bot 82:219–227CrossRefGoogle Scholar
  6. Huang CC, Chiang TY, Hsu TW (2007) Isolation and characterisation of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA). Conserv Genet. doi: 10.1007/s10592-0079341-z
  7. Lewandowski A, Burczyk J, Mejnartowicz L (1995) Genetic structure of English yew (Taxus baccata L.) in the Wierzchlas Reserve: implications for genetic conservation. For Ecol Manag 73:221–227CrossRefGoogle Scholar
  8. Paule L, Gömöry D, Longauer R (1993) Present distribution and ecological conditions of the English yew (Taxus baccata L.) in Europe. In: International yew resources conference: yew (Taxus) conservation biology and interaction. Berkeley, CA, pp 189–196Google Scholar
  9. Rousset F (2007) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Notes. doi: 10.1111/j.1471-8286.2007.01931.x
  10. Rozen S, Skaletsky HJ (2000) PRIMER 3 on the WWW for general users and for biologist programmers. In: Bioinformatics methods and protocols: methods in molecular biology. Totowa, NJ: Humana Press, pp 365–386. Source code available at
  11. Thomas PA, Polwart A (2003) Biological flora of the British Isles. J Ecol 91:489–524CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Marta Dubreuil
    • 1
  • Federico Sebastiani
    • 2
  • Maria Mayol
    • 1
  • Santiago C. González-Martínez
    • 3
  • Miquel Riba
    • 1
  • Giovanni G. Vendramin
    • 4
  1. 1.CREAF (Center for Ecological Research and Forestry Applications)Autonomous University of BarcelonaBellaterraSpain
  2. 2.Department of Agricultural BiotechnologyGenExpress, University of FlorenceSesto FiorentinoItaly
  3. 3.CIFOR-INIA (Center of Forest Research)MadridSpain
  4. 4.Plant Genetics Institute, National Research CouncilSesto FiorentinoItaly

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