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Conservation Genetics

, Volume 9, Issue 6, pp 1661–1663 | Cite as

Isolation and characterization of microsatellite loci in the Brazilian orchid Epidendrum fulgens

  • F. PinheiroEmail author
  • M. O. Santos
  • F. Barros
  • D. Meyer
  • A. Salatino
  • A. P. Souza
  • S. Cozzolino
Technical Note

Abstract

Epidendrum fulgens has a patchy distribution along the Atlantic Rainforest in the Brazilian coast, due to the destruction of its native habitat. Here, we report on both the development of nine new microsatellite markers isolated from this species and the characterization of their allele variability in two distant and unrelated populations. The number of alleles observed for each locus ranged from 2 to 17 with an average of 6.4 alleles per locus. These microsatellites should be valuable tools for studying the effect of habitat fragmentation on the genetic structure of E. fulgens populations.

Keywords

Epidendrum Orchidaceae Microsatellites Atlantic Rainforest Cross-amplification 

Notes

Acknowledgements

We thank C. Palma-Silva and R. P. Romanini for technical support and helpful discussions on this manuscript. This work was supported by grants from FAPESP (06/54189-3) and CNPq (471929/2006-9) to F. Pinheiro and F. Barros, and by awards from CAPES to F. Pinheiro and CNPQ to F. Pinheiro, F. Barros, A. Salatino and A. P. Souza.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • F. Pinheiro
    • 1
    • 2
    Email author
  • M. O. Santos
    • 3
    • 4
  • F. Barros
    • 5
  • D. Meyer
    • 6
  • A. Salatino
    • 1
  • A. P. Souza
    • 3
    • 4
  • S. Cozzolino
    • 2
  1. 1.Departamento de BotânicaIB/USPSao PauloBrazil
  2. 2.Dipartimento delle Scienze BiologicheUniversità degli Studi Federico IINaplesItaly
  3. 3.Departamento de Genética e EvoluçãoIB/UNICAMPCampinasBrazil
  4. 4.Centro de Biologia Molecular e Engenharia Genética (CBMEG)UNICAMPCampinasBrazil
  5. 5.Instituto de BotânicaSeção de Curadoria do HerbárioSao PauloBrazil
  6. 6.Departamento de Genética e Biologia EvolutivaIB/USPSao PauloBrazil

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